2D Tumor Modeling Services

Two-dimensional (2D) tumor models have long been a cornerstone of cancer research, providing researchers with a convenient and cost-effective platform for studying fundamental aspects of tumor biology and evaluating potential therapeutic interventions. At Alfa Cytology, we leverage our expertise and cutting-edge technologies to develop highly reproducible and reliable 2D tumor models that enable researchers to gain valuable insights into tumor initiation, progression, and response to treatment.

Introduction into 2D Tumor Modeling

Traditional 2D cell culture systems involve the growth of tumor cells on flat surfaces, such as tissue culture plates or flasks, in a monolayer format. While simplistic in nature, these models offer several advantages, including ease of use, scalability, and cost-effectiveness. They provide researchers with a controlled environment to study key aspects of tumor biology, including cell proliferation, migration, invasion, and response to therapeutic agents.

Fig.1 2D cell culture. (Bou-Ghannam, S., et al., 2021)

Commonly Used 2D Cancer Models

• Monolayer Cell Cultures: Cancer cells grown as a single layer on tissue culture plates or dishes.
• Transwell Assays: Utilizes porous membrane inserts to study cell migration, invasion, and interaction between different cell populations.
• Scratch Assays: Involves creating a "scratch" or wound in a monolayer of cells to study cell migration and wound healing.
• Clonogenic Assays: Measures the ability of single cancer cells to form colonies when plated at low densities.
• Soft Agar Assays: Evaluates the anchorage-independent growth of cancer cells in a semi-solid agar matrix, mimicking tumorigenicity in vitro.

Our Services

Our 2D Tumor Modeling Services cater to a wide range of research applications, from basic mechanistic studies to high-throughput drug screening assays. By leveraging optimized protocols and quality-controlled processes, we ensure the reproducibility and reliability of our 2D tumor models, allowing researchers to obtain accurate and interpretable data for their research projects.

Workflow of Our Services

• Consultation and Project Design: We collaborate closely with clients to understand their research goals, experimental design, and technical specifications. This initial consultation allows us to tailor our services to meet the specific needs and objectives of each project.
• Cell Culture and Model Development: We culture tumor cells in a monolayer format using optimized culture conditions and protocols. Our team monitors cell growth and morphology throughout the culture period to ensure the successful generation of 2D tumor models.
• Experimental Assays and Data Analysis: We perform a variety of experimental assays, including proliferation assays, migration assays, invasion assays, and drug screening assays, to characterize the phenotypic and functional properties of our 2D tumor models. Comprehensive data analysis and interpretation services are provided to help clients extract meaningful insights from their experiments.

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The Advantages of Our Service

Alfa Cytology is a leader in cancer modeling and translational research, dedicated to advancing the understanding and treatment of cancer through innovative and cutting-edge technologies. Our multidisciplinary team of experts, state-of-the-art facilities, and commitment to excellence enable us to deliver reliable, reproducible, and high-quality 3D tumor models for a wide range of research applications. Contact us today to learn more about our 3D Tumor Modeling Services and how we can support your research goals.

! For research use only.