Antibody Characterization Service
Therapeutic antibodies play a crucial role in the treatment of various diseases, especially cancer. The effectiveness of an antibody therapy depends on its ability to correctly recognize the target antigen with optimal affinity and a high specificity. Alfa Cytology offers an antibody characterization service that provides clients with intrinsic insights into specificity, mechanism of action, and safety.
Importance of Antibody Characterization
Antibody characterization is a key process in the development and study of therapeutic antibodies and is essential to ensure the quality, specificity, functionality, and safety of antibodies, helping researchers to make informed decisions about antibody selection, optimization, and application.
Quality Control
Antibody characterization ensures the quality and consistency of antibodies for research, diagnostic, and therapeutic use. It helps to detect variants or impurities that may affect the performance and reliability of the antibody.
Specificity and Selectivity
Characterization confirms the specificity and selectivity of an antibody for a particular target. It helps to determine whether the antibody can accurately and efficiently bind to the target molecule without cross-reacting with irrelevant molecules.
Functional Validation
Antibody characterization assesses the function of an antibody, including its ability to neutralize, block, activate, or modulate specific biological pathways, which ensures that the antibody is functional and appropriate for its intended application.
Binding Affinity and Kinetics
Characterization provides information about the binding affinity and kinetics, such as the rate of binding and dissociation. These data are important for understanding an antibody's binding strength, stability, and duration of binding to a target molecule.
Epitope Mapping
Antibody characterization helps to identify and map the epitopes recognized by the antibody. This information is valuable for understanding an antibody's mechanism of action and potential applications in diagnostics, therapy, or research.
Safety
Antibody characterization is important for assessing safety parameters such as immunogenicity, cytotoxicity, and potential side effects. These assessments are critical for regulatory compliance and to ensure the safety of antibody products for subsequent human or animal use.
Our Services
Antibody characterization services are essential for the development of the most promising therapeutic antibody candidates for cancer. Alfa Cytology's antibody characterization service provides a complete biophysical profile of your key candidates and is designed to save you time by eliminating underperforming candidates early in the discovery process.
Target-expressing Cell Model Construction Services
The construction of relevant cell lines expressing target antigens with accurate expression levels helps to further proceed with antibody characterization services, laying the foundation for successful downstream antibody validation, characterization, and functional studies, with minimal false positives and false negatives.
Antibody Target Specific Binding Assessment
Our specific binding assessment service helps clients analyze receptor binding relationships to identify the most effective candidates and gain insight into how candidate antibodies behave in complex systems.
Target Binding Affinity - We offer a variety of methods to assess target binding based on your needs.
Biochemical Characterization - We offer nM range sensitivity and rapid cell-free solution assays to help understand various interactions.
EC 50 Binding Potency Assessment - Ranking of cell lines and antibody candidates based on their binding profiles.
Mechanistic Studies
The functional relevance of an antibody binding to its target is important for assessing the potential therapeutic effect of the candidate. We provide assays to support the characterization services of candidate antibodies. Our mechanism studies include but are not limited to the following.
- Activation or inhibition of enzyme activity
- Antibody-dependent cytotoxicity (ADCC)
- Activation or inhibition of signaling pathways
- Antibody-dependent cell phagocytosis (ADCP)
- Direct killing of target cells (apoptosis)
- Complement-dependent cytotoxicity (CDC)
One-step Antibody Development
Our services in antibody development extend beyond antibody characterization services. Alfa Cytology is dedicated to the development of a wide range of therapeutic antibodies against cancer, helping you to develop antibody therapeutics from the ground up.
Alfa Cytology's antibody characterization service is the next step after antibody discovery and is also suitable for researchers wishing to characterize antibodies in existing cell lines. We are confident to provide you with technical support for drug discovery, manufacturing processes, and other special requirements. Please contact us for more information.
! For research use only.
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