Antibody Discovery Service
Antibodies are versatile molecular binding agents with an established and growing role as therapeutic agents, which can provide significant benefits in cancer therapy. Alfa Cytology offers therapeutic antibody discovery services, providing clients with new directions in therapeutic antibody design.
Fig.1 Approaches for the development of therapeutic antibodies. (Lu, R. M., et al., 2020)
Technologies for Antibody Discovery
With the continuous development of antibody engineering, the currently developed antibody drugs have fewer and fewer adverse reactions due to their high specificity. Therefore, therapeutic antibodies have become a major class of new drugs developed for cancer therapy in recent years. Nowadays, the superior antibody engineering techniques used to develop therapeutic antibody drugs mainly include the mouse hybridoma technique, phage display, transgenic mouse, and single B cell technique.
Mouse Hybridoma Technique
This method involves fusing antibody-producing B cells from immunized animals with immortalized myeloma cells to create hybridoma cell lines to produce monoclonal antibodies (mAbs) with high specificity for the target antigen.
Phage Display
A library of antibody fragments is displayed on the surface of phages. The phage library is then screened against the target antigen to identify phage clones expressing antigen-specific antibodies.
Transgenic Mouse
Transgenic or humanized mice can be engineered to express human antibody genes, allowing the generation of fully human antibodies.
Single B Cell Sorting
This method involves isolating individual B cells from immunized animals or human donors and screening them for antigen-specific antibodies.
Our Services
Alfa Cytology offers comprehensive solutions tailored to your unique vision for your next antibody therapy. Our goal is to provide rapid and robust results to support the success of your antibody candidate.
Comprehensive Antibody Discovery Platform
Achieve robust and rapid antibody discovery from our comprehensive antibody discovery platform. Alfa Cytology's antibody discovery platform integrates mouse hybridoma technique, phage display, transgenic mouse, and single B cell technique. We design the antibody candidates with therapeutic exploitability in mind, tailoring them to your unique vision.
Powerful Antibody Discovery Capabilities
Alfa Cytology can provide antibody discovery services with different types of antibodies to promote your specific cancer therapy development projects.
One-step Antibody Development
Our experienced antibody discovery team can provide you with a one-step antibody discovery service that leverages our expertise to drive your project forward with unprecedented support, speed, and customizability. Notably, our antibody specificity and off-target screening services assess antibody specificity and potential off-target binding interactions.
Alfa Cytology uses a variety of techniques such as ELISA, western blotting, immunohistochemistry, and flow cytometry to assess antibody specificity and help clients ensure the accuracy and reliability of antibody candidate-based therapeutic and diagnostic applications. In addition, we provide in vivo efficacy and safety services through PK/PD studies and early safety assessments.
Workflow of Therapeutic Monoclonal Antibody Development
Target Identification
Identifying potential target molecules that are involved in the disease pathway through genomics and proteomics.
01
Target Validation
The identified targets are further validated to ensure their relevance and suitability as therapeutic targets.
02
Antibody Generation
Generation of specific anti-cancer antibodies using hybridoma technology, phage display, and transgenic animals.
03
Lead Antibody Selection
Functional assays and engineering techniques are applied to optimize their therapeutic potential.
04
Preclinical Studies
Efficacy, safety, pharmacokinetics, and pharmacodynamics are tested in relevant disease models.
05
Alfa Cytology's antibody discovery experience provides you with dedicated antibody discovery and development expertise to guide you through antibody therapy development with unprecedented support, speed, and customizability. Start your journey with a partner who understands drug discovery and can put you at ease. Please contact us for more information.
Reference
-
Lu, R. M., et al.; (2020). Development of therapeutic antibodies for the treatment of diseases. Journal of biomedical science, 27, 1-30.
For research use only.
Related Services
