Calcium Probe Neural Stem Cell Lines

Changes in calcium ion (Ca²⁺) concentration are an important mode of information transfer in the cascade of responses that occur in living cells after receiving external stimuli. Therefore, intracellular Ca²⁺ detection is an essential research tool in signal transduction G protein-coupled receptor (GPCR)-mediated screening of relevant brain tumor drugs and other related experiments.

We offer various Ca²⁺ probe-based neural stem cells for development and related products

Ca²⁺ imaging is widely used in the fields of neurobiology, cell biology, physiology, developmental biology, and pharmacology. Alfa Cytology uses indicators that can sense Ca²⁺ concentration to convert changes in calcium concentration into fluorescent signals, and the displayed spectra can be detected by a variety of methods such as fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence enzyme markers. In brain tumor and neurobiology, you can study the signal emission triggered by the change of ionic concentration in neuronal cells by using the Ca²⁺ probe-like neural stem cells developed by us, and determine the activity of neuronal cells by recording the presence and intensity of luminescence of the neuronal cells labeled with GFP.

Intracellular Ca²⁺ is involved in the facilitation of inter-synaptic signaling in neurons, and the level of intracellular Ca²⁺ concentration detected by calcium imaging can reflect the function of neuron-like cells. Two types of Ca²⁺ indicators, chemical indicators, and genetically encoded indicators are now commonly used, such as GCaMP. GCaMP consists of circularly permuted green fluorescent proteins (cpGFP), calmodulin (CaM), and a peptide sequence M13 of myosin light chain kinase. When Ca²⁺ binds CaM, CaM conformationally changes and its hinge region binds M13, causing cpGFP to fluoresce intensely with increased protonation of its chromophore and an increase in absorbance in the presence of light at specific wavelengths. 300s after activation of the indicator, the fluorescent signal filled the whole cell, including dendrites and dendritic spines.

Developable calcium probe-like neural stem cells

Transplantation of stem cells into diseased or injured brain tissue can generate new nerve cells to repair and improve the nervous system structurally and functionally. Alfa Cytology offers a wide range of Ca²⁺ probe-based neural stem cell development and related products as a biotherapeutic approach to help you in your pioneering exploration in the field of brain tumors. Please feel free to contact us for protocol and product information.