Cytokine Analysis Services for Brain Tumors

Cytokines and their receptors play a key role in biological processes and are essential mediators in cell communication within the tumor microenvironment. Some cytokines can contribute to host antitumor responses while some may promote cancer advancement when dysregulated. At Alfa Cytology, we offer a variety of cytokine analysis services tailored to brain tumor research.

Cytokines in Brain Tumors

Cytokines are small peptides and proteins synthesized and secreted by immune and non-immune cells, which can regulate cellular signaling and immune response within the tumor microenvironment. Cytokines play a dual role in brain tumor progression. Some cytokines like IL-2 can support brain antitumor responses while others such as IL-1β and IL-6 contribute to brain tumor growth and therapy resistance when dysregulated. Hence, a comprehensive understanding of cytokines is crucial for advancing brain tumor research and the development of potential therapeutics.

Fig.1 Protumoral and antitumoral roles of type I IFNs in the glioma tumor microenvironment. (Lim J., et al., 2023)

Our Services

At Alfa Cytology, we specialize in employing proven methods to analyze a wide array of cytokines including interferons, interleukins, lymphokines and tumor necrosis factors, offering precise qualitative and quantitative analyses of brain tumor-related cytokines. Our analysis services include cell biology assays, molecular biology assays and immunology assays.

Types	Assays
By methods	Singleplex cytokine assay. Multiplex cytokine assay. Cytokine release syndrome (CRS) assay. Intracellular cytokine assay.
By application	Cytokine signaling pathway analysis. Immune response analysis. Biomarker Identification and validation assays. Drug efficacy validation analysis.

Our capabilities extend to detecting multiple cytokines within a single sample. We accept a variety of sample types for testing, such as plasma, urine, cerebrospinal fluid, cell lysates and tissue lysates.

Cytokine Analysis Methods

Multiple cytokine analysis methods including enzyme-linked immunosorbent assay (ELISA), cytometric bead array (CBA) and intracellular cytokine staining combined with flow cytometry are employed to accurately analyze various cytokines. With our advanced technologies, we are committed to helping you analyze various cytokines to address your diverse requirements.

Fig.2 ELISA.

Enzyme-linked Immunosorbent Assay (ELISA)

Fig.3 Cytometric bead array.

Cytometric Bead Array (CBA)

Fig.4 Intracellular cytokine staining.

Intracellular Cytokine Staining (ICS)

Workflow of Cytokine Analysis

Customer Inquiry

Project Initiation

Method Development

Sample Measurement

Data Analysis

Advantages of Our Cytokine Analysis

Rapid quantification of multiple cytokines simultaneously.
Reduce workflow and sample volume requirements by combining the analysis of multiple target analytes in a single reaction volume.
Quantify targets at different concentrations by providing linearity over a wide range.

Contact Us

Understanding how cytokines influence and regulate tumor cell communication can help improve your diagnostic capabilities and facilitate therapeutic advancement. Alfa Cytology is an industry pioneer in cytokine detection. Our established and reliable cytokine analysis services are of high sensitivity and good reproducibility and are tailored specifically for your brain tumor research. Please feel free to contact us to discuss any project regarding cytokine analysis for brain tumors.

Reference

Lim J.; et al. (2023). Harnessing type I interferon-mediated immunity to target malignant brain tumors[J]. Frontiers in immunology. 14, 1203929.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.