Galc Polyclonal Antibody

CAT#: OLD-D002

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Overview Specification

Target	GALC
Cell Type	Oligodendrocytes
Host	Rabbit
Isotype	IgG
Applications	WB, IP, IHC, IF, ELISA
Reactivity	Human, Mouse, Rat, Zebrafish
Conjugate	Unconjugated
Keywords	Oligodendrocytes; Rabbit; IgG; WB, IP, IHC, IF, ELISA

Product Information	It targets Galc in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Background Information	The GALC antibody targets the liposomal enzyme Galactosylceramidase (GALC), which belongs to the glycosyl hydrolase 59 family. It hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. It is primarily found in the brain and kidneys where galactolipids are hydrolyzed (PMID:8634707). Deficiencies of GALC are primarily associated with the autosomal recessive Krabbe's disease. This disease is characterized by developmental delay caused by apoptosis of myelin-forming cells. GALC is responsible for hydrolyzing galactosylceramide, a cerebroside that is an important component of myelin. A deficiency in GALC causes loss of myelin to nerve cells, resulting in delayed nerve transmissions. Krabbe's disease has varying degrees of severity due to a large number of different genetic mutations in the gene. The GALC antibody can be used to detect the deletions in the GALC gene and functions of the enzyme (PMID:20886637). Normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits (PMID: 26865610).
Full Name	Galactosylceramidase
Synonyms	2310068B06Rik, A930008M05Rik, AW212969, AW413532, Gacy, galactosylceramidase, Galc, twi, twitcher
Tested Reactivity	Human, mouse, rat
Cited Reactivity	Human, mouse, zebrafish
Class	Polyclonal
Type	Antibody
Immunogen	Galc fusion protein
Calculated Molecular Weight	77 kDa
Observed Molecular Weight	80 kDa, 30 kDa, 50 kDa
Gene ID	14420
GenBank Accession Number	BC086671
Gene Symbol	Galc
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
Positive WB Detected	A375 cells, A549 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IP Detected	NIH/3T3 cells
Positive IHC Detected	Human gliomas tissue

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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