Gene Microarray Analysis for Brain Tumors
The fatal drawback of in situ hybridization and NORTHERN techniques is the extremely low throughput, i.e. only a limited number of genes can be detected in a single experimental procedure. If one wants to detect the pattern of changes of numerous genes in a specific experimental system in a limited number of experiments, these two techniques are not possible. And this can be achieved using microarrays. Traditional methods for detecting many genes require multiple experiments and low automation, and thus there is a systematic error between each experiment. Gene microarrays overcome this disadvantage, as the labeling and hybridization of probes for many genes are done in a single experiment, with a high degree of automation and objective and reliable data.
Gene microarray analysis services we provide for brain tumor
Alfa Cytology uses gene microarray technology and bioinformatics analysis to screen for core genes (differentially expressed genes) and signaling pathways associated with brain tumors, including glioblastoma multiforme. We can also perform clustering analysis and functional enrichment analysis of differential genes while constructing protein-protein interactions (PPI) networks to screen core genes. Finally, we validate the expression levels of the core genes with database and genome-wide expression profile data. This allows us to provide you with more possible molecular targets for the potential diagnosis and treatment of brain tumors.
Brain tumor gene microarray preparation
| Methods |
Details |
| In situ synthesis method |
We can use solid-phase chemistry, photoliabile protecting groups, and photobithography to obtain a collection of well-located, highly diverse compounds. We can make a large number of different probes in a small area.
In addition, we have the equipment to prepare chips with standard phosphoramidite reagents, which can be moved throughout the chip by the chip printhead, and specific bases can be printed at specific positions on the chip, depending on the sequence of the probes at different sites. No special chemical reagents are required in this process, and the efficiency of each synthesis step is up to 98%, with synthetic oligonucleotides of 40-50 nt in length. |
| Post-synthesis attachment method |
We use manual or automated spotting devices to spot pre-prepared oligonucleotide or cDNA samples on specially treated glass sheets or other materials. And for different lengths of nucleic acid molecules, we use different treatments for the solid surface. |
Brain tumor gene microarray data analysis
Gene microarrays can scan the whole genome at the molecular level and play an important role in brain tumor diagnosis and treatment. Alfa Cytology uses gene microarrays to predict the risk of brain tumors in high-risk susceptible populations, to make early, staging, prognosis, and differential diagnoses of brain tumors, and to clearly distinguish tumor subtypes. We are also able to monitor multiple viruses associated with brain tumors, which is essential for brain tumor prediction, diagnosis, and treatment. Please contact our staff for a customized gene chip for your project to assist you in achieving breakthroughs in brain tumor research.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
