GLAST Polyclonal Antibody

CAT#: AST-M003

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Overview Specification

Target	GLAST
Cell Type	Type 2 astrocytes
Host	Rabbit
Isotype	IgG
Applications	WB, IP, IHC, IF, ELISA
Reactivity	Human, mouse, rat, cow
Conjugate	Unconjugated
Keywords	Type 2 astrocytes; Rabbit; IgG; WB, IP, IHC, IF, ELISA

Product Information	The immunogen of GLAST Fusion Protein expressed in E. coli.
Background Information	SLC1A3, also known as EAAT-1 or GLAST, is a membrane-bound protein localized in glial cells and pre-synaptic glutamatergic nerve endings. It transports the excitatory neurotransmitters L-glutamate and D-aspartate, which is essential for terminating the postsynaptic acction of glutamate. Recently, a correlation between expression/function of glial EAAT-1 and tumor proliferation has been reported. The exceptionally rare expression of EAAT-1 in non-neoplastic choroid plexus (CP) compared to choroid plexus tumors (CPT) may distinguishes neoplastic from normal CP. There are a number of splicing variants of SLC1A3, like GLAST1a and GLAST1b, exist due to the exon skipping. It also undergo glycosylation. Variety of bands can be observed in the western blotting assay: 50-55 kDa represents the unglycosylated GLAST1a or GLAST1b, 65-70 kDa correspond to the glycosylated proteins, larger proteins between 90-130 kDa may be the multimers of SLC1A3.
Synonyms	EA6, EAAT1, GLAST, GLAST 1, GLAST1, SLC1A3
Full Name	Solute carrier family 1 (glial high affinity glutamate transporter), member 3
Tested Reactivity	Human, mouse, rat
Cited Reactivity	Human, mouse, rat, cow
Type	Antibody
Class	Polyclonal
Immunogen	GLAST fusion protein
Calculated Molecular Weight	542 aa, 60 kDa
Observed Molecular Weight	50-55 kDa, 90-100 kDa
GenBank Accession Number	BC037310
Gene ID	6507
Gene Symbol	SLC1A3
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
Positive WB Detected	Neuro-2a cells, C6 cells, mouse brain tissue
Positive IP Detected	Mouse brain tissue
Positive IHC Detected	Human brain tissue, mouse brain tissue

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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