GPCRs Targeted Drug Development Services for Brain Tumors

G protein-coupled receptors (GPCRs) are widely found in animals and are the largest family of membrane receptor proteins in the human body. They are widely involved in the regulation of cell proliferation, differentiation, migration, and other physiological activities. The structural features and important role in signaling determine that they can be good drug targets for brain tumor drug screening.

We provide GPCRs targeted drug screening services for brain tumors

Alfa Cytology can provide GPCRs targeting screening and discovery services to our clients. We proactively understand our client's specific requirements for brain tumor drug development and facilitate their research and product development, combining years of experience in design, development, and data to deliver satisfactory results for our clients. We can provide a full suite of R&D services, including the construction of stable cell lines for GPCRs, cell membrane preparation, Binding assay, calcium flow, second messengers (cAMP, IP-One), reporter genes, and many other assays. We provide not only high-quality screening model construction services, but also high-throughput screening and compound profiling services to our customers.

• GPCRs binding assays. We can perform ligand binding assays including radiolabeled ligand binding assays and other fluorescent-labeled ligand binding assays to detect the binding ability of compounds to target targets.
• GPCRs functional analysis assays. We can perform functional assays, including cAMP and Ca2+ assays, to analyze the activation and inhibition of compounds on target sites.

General service flow

The GPCRs-targeted drug screening platform

• Radioisotope labeled GPCRs ligand binding assay or GTPrS binding assay.
• Downstream cAMP signal assay or homogeneous time-resolved fluorescence assay for intracellular GPCRs activation.
• Calcium flow assay triggered by activation of intracellular GPCRs.
• Detection of downstream phosphatidylinositol (IP3) activated by intracellular GPCRs.
• Protein phosphorylation assay.
• Reporter gene-based assays.

Sample requirements

Customers only need to provide the compound of the target to be tested, and we will arrange the corresponding screening and testing services for you, providing you with a professional identification report.

Our advantages

• Constructed cell lines with over 100 GPCR targets.
• Multiple compounds can be screened simultaneously at one time.
• Test flexibility, can provide target-compound binding assays and functional analysis services separately, and provide single concentration or concentration gradient IC50 assay services.
• We provide agonist, antagonist, inverse agonist, and transmodulation screening and evaluation for different types of targets.
• High throughput screening platform based on calcium flow, cAMP quantification, and reporter gene style.

Alfa Cytology has developed rigorously tested SOPs for screening conditions in all aspects of GPCRs to provide you with high-quality service and high-quality results through experimental services to accelerate our clients' new drug discovery and development in brain tumors. Please contact us to submit your requirements.