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Human NFH Protein, His Tag

Human NFH Protein, His Tag

CAT#: IVD-P020

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Overview Specification

Species	Human
Tag	His
Expression Systems	Baculovirus-Insect cells
Keywords	In vitro diagnostic proteins

Synonyms	Neurofilament heavy polypeptide
Background	Neurofilaments (NF) are classed as type IV intermediate filaments found in the cytoplasm of neurons. Together with microtubules and microfilaments, they form the neuronal cytoskeleton. Neurofilaments usually contain three intermediate filament proteins: NFL, NFM, and NFH which are involved in the maintenance of neuronal caliber. Neurofilament light (NfL) is a scaffolding protein that is located primarily within myelinated axons and that provides increased conduction speed and structural support. In recent years, NfL has been used as a disease biomarker on the basis of the observation that axonal injury results in elevated levels of NfL in cerebrospinal fluid or blood.
Source	Human NFH
SWISS	P12036-1
Theoretical Molecular Weight	114.5 kDa
Formulation	Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with trehalose as protectant.
Reconstitution	Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Molecular Characterization	This protein carries a polyhistidine tag at the N-terminus. The protein migrates as 160-230 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin	Less than 1.0 EU per μg by the LAL method.
Purity	>95% as determined by SDS-PAGE.
Storage	For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Notes	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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