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Human Recombinant A53T Mutant Alpha Synuclein Protein Pre-formed Fibrils (Type 1)

Human Recombinant A53T Mutant Alpha Synuclein Protein Pre-formed Fibrils (Type 1)

CAT#: PFF-S015

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Overview Specification
Species Human
Tag Tag Free
Expression Systems E.coli
Keywords Preformed fibrils
Synonyms A53T mutant alpha synuclein, A53T mutated SNCA, A53T Alpha synuclein PFFs, Alpha synuclein PFF, Ala53thr mutant alpha synuclein, Alpha synuclein pre-formed fibrils, Alpha synuclein aggregates, Alpha synuclein protein aggregates, Alpha synuclein aggregates, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, SYN protein, Parkinson disease familial 1 Protein
Background Alpha-synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals. Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum, and thalamus. Functionally, it has been shown to significantly interact with tubulin and may serve as a potential microtubule-associated protein. SNCA fibrillar aggregates represent the major non-A-beta component of Alzheimer's disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin. The A53T mutation is a missense point mutation where alanine is replaced by threonine at the 53rd amino acid. This mutation has been linked to early-onset Parkinson's Disease and increased rates of alpha-synuclein fibrillization.
Description Alpha Synuclein Protein
SWISS P37840
Accession Number NP_000336.1
Gene ID 6622
Cellular Localization Cytoplasm, Membrane, Nucleus
Amino Acid Sequence MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
Protein Length Full length
Applications WB, SDS-PAGE, In vivo assay, In vitro assay
Research Areas Alzheimer's Disease, Neurodegeneration, Neuroscience, Parkinson's Disease, Synuclein, Tangles & Tau, Multiple System Atrophy
Concentration Lot/batch specific. See included datasheet.
Nature Recombinant
Biological Activity 100 µM A53T alpha synuclein protein monomer seeded with 10 uM A53T alpha synuclein protein PFF in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated a fluorescence intensity of 28,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 56 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
Storage Buffer 1X PBS pH 7.4
Purity >95%
Purification Ion-exchange Purified
Storage Stored at -80°C
Shipping Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.
Notes Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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