Human Recombinant Alpha Synuclein Pre-formed Fibrils (Type 2)

CAT#: PFF-S022

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Overview Specification

Species	Human
Tag	Tag Free
Expression Systems	E.coli
Keywords	Preformed fibrils

Synonyms	Alpha synuclein PFFs, Alpha synuclein PFF, Alpha synuclein aggregates, Alpha synuclein protein aggregates, Alpha synuclein aggregates, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, SYN protein, Parkinson disease familial 1 Protein
Background	Alpha-synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals. Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum, and thalamus. Functionally, it has been shown to significantly interact with tubulin and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of cognitive functions; inactivation may lead to impaired spatial learning and working memory. SNCA fibrillar aggregates represent the major non-A-beta component of Alzheimer's disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin.
Description	Alpha Synuclein Protein
SWISS	P37840
Accession Number	NP_000336.1
Gene ID	6622
Cellular Localization	Cytoplasm, Membrane, Nucleus
Amino Acid Sequence	MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
Protein Length	Full length
Applications	WB, SDS-PAGE, In vivo assay, In vitro assay
Research Areas	Alzheimer's Disease, Neurodegeneration, Neuroscience, Parkinson's Disease, Synuclein, Tangles & Tau, Multiple System Atrophy
Concentration	Lot/batch specific. See included datasheet.
Nature	Recombinant
Biological Activity	Does not induce Lewy body inclusion formation in Sprague-Dawley rat primary hippocampal neurons. Thioflavin T emission curve shows only a small increase in fluorescence (indicative of alpha synuclein aggregation) when Type 2 alpha synuclein PFFs are combined with alpha synuclein monomers. Certain biological activities in other neuronal cells cannot be ruled out. Researchers should test compatibility prior to use.
Storage Buffer	1X PBS pH 7.4
Purity	>95%
Purification	Ion-exchange Purified
Storage	Stored at -80°C
Shipping	Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.
Notes	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

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