Methylation Sequencing for Brain Tumors
As a central component of epigenetics, DNA methylation is critical for maintaining normal cell function, embryonic development and various physiological processes. Aberrations in DNA methylation play a significant role in tumorigenesis and metastasis. Alfa Cytology offers comprehensive genome-wide DNA methylation detection and analysis to investigate the intricacies of brain tumor progression, metastasis and recurrence, aiming to promote the development of innovative anti-brain tumor strategies.
Methylation Sequencing in Brain Tumors
DNA methylation is an important epigenetic modification that governs gene expression and genome stability. Dysregulation of DNA methylation is intricately related to a variety of diseases such as brain tumors. The demethylation of extensive genomic regions and the hypermethylation of CpG islands are recognized as cancer cell hallmarks. Therefore, DNA methylation signature-based analysis has become a vital tool in brain tumor research. With the evolution of next-generation sequencing, diverse methodologies have been introduced to scrutinize DNA methylation abnormalities involved in brain tumors.
Fig.1 The evolution of DNA methylation profiling methodology. (Li S., & Tollefsbol TO, 2021)
Our Services
Alfa Cytology offers methylation sequencing services to provide our clients with detailed methylation profiles of brain tumors. Our services can be integrated with standard techniques such as histology, enhancing the effectiveness of DNA methylation analysis as a valuable complement to brain tumor classification, especially for rare and poorly characterized brain tumors.
Whole genome bisulfite sequencing (WGBS)
Achieve single-base resolution, facilitating analysis of the methylation status of each cytosine and generating a detailed genome-wide DNA methylation map.
Reduced representation bisulfite sequencing (RRBS)
Detect the methylation status of CG-rich regions, primarily regulatory regions within the genome.
Bisulfite amplicon sequencing (BSAS)
Employ next-generation sequencing for efficient high-throughput analysis after bisulfite treatment and PCR amplification of specific target regions.
Targeted bisulfite sequencing
Utilize specific primers to capture regions of interest and assess site-specific DNA methylation alterations.
Methylation-specific PCR (MSP)
Utilize targeted PCR amplification of bisulfite-converted DNA to precisely detect DNA methylation across nearly any group of CpG sites.
Workflow of Methylation Sequencing
Sample Preparation
Methylation Sequencing
Library Construction
Data Analysis
Data Analysis Content
Statistical Data Comparisons
- Data quality control
- Output statistics
Methvlation Statistics of Individual Samples
- Chromosome methylation statistics
- Genomicmethylation loop map
- Gene methvlation map
Differentially Methylated Regions(DMRs)Analysis
- Gene-based DMRs analysis
- miRNA-based DMRs analysis
- Repeat sequence DMRs analysis
Our Services Help You Achieve the Following Goals
- Comprehensively analyze treatment effects on genome-wide methylation levels and quickly identify differentially methylated regions.
- Provide insights into brain tumor tissue origin and histological subtypes. Utilize tumor tissue DNA methylation sequencing data to identify and catalog nearly 100 distinct brain tumors.
Advantages of Methylation Sequencing
- Enhanced Library Quality. Based on years of experience and expertise, we excel in sample processing and library construction, ensuring reliable library quality through our methylation sequencing services.
- Comprehensive Quality Control. Our rigorous quality control procedures encompass a full range of analyses to guarantee data precision and integrity.
- Clear and Informative Data Reports. Our data reports are designed for clarity, featuring comprehensive graphical content and detailed manuals to empower clients to interpret and utilize the data effectively.
- Tailored Bioinformatics Support. With an experienced bioinformatics team proficient in diverse analysis tools, we offer personalized analysis concepts and solutions, collaborating closely with clients for optimal outcomes.
Contact Us
Alfa Cytology provides methylation sequencing services to illuminate variations in genomic methylation levels in brain tumors, aiming to unravel the molecular mechanisms of brain tumorigenesis and identify novel markers for early tumor detection. Please contact us for further details on our DNA methylation sequencing services.
Reference
- Li S., & Tollefsbol TO. (2021). DNA methylation methods: Global DNA methylation and methylomic analyses[J]. Methods (San Diego, Calif.). 187, 28–43.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
