Nanopore Sequencing for Brain Tumors

Based on its extremely high spatial resolution and more intuitive detection principle, nanopore technology has formed a more complete system and unique function of single-molecule biophysical tools. Nanopore sequencing is the most important application of nanopore technology and the most representative single-molecule sequencing tool or third-generation sequencing tool. Real-time nanopore sequencing allows comprehensive analysis of clinical research samples and rapid access to information about the brain tumor research field.

Our nanopore sequencing services for brain tumors

Alfa Cytology offers nanopore sequencing services for the complete characterization of structural variants (SV), repeat regions, single nucleotide variants (SNV), haplotype fixation, RNA splice variants, fusion transcripts, and epigenetic modifications. We master the size and surface properties of nanopores and applied voltages and solution conditions to detect different types of biomolecules.

Basic services flow

In addition, we can scan and read the amino acid sequences of individual proteins using nanopore sequencing technology. Linearized DNA-peptide complexes slowly pass through tiny nanopores and depending on the variation and intensity of the current, we can read the relevant protein information content and sequence the amino acid sequence of the protein directly. We can detect small protein variation changes and can distinguish individual amino acid changes with high fidelity and high throughput potential. It can provide a new path for sequencing and classification of single-molecule proteins within single cells and the detection and treatment of brain tumors.

Our advantages

• Low cost, high throughput, non-labeling.
• The single-molecule resolution, long read length, portable operation, and can be coupled with other sequencing methods.
• Ability to directly read out methylated cytosine (C), which can be used to detect the cellular origin of cell-free DNA (cfDNA) and brain tumor-specific methylation profiles.
• Streamlined workflow with rapid library preparation and real-time analysis.
• Real-time data streaming helps you get results quickly.

Alfa Cytology can utilize nanopore DNA methylation analysis (NDMA) for rapid, detailed brain tumor typing. We can also perform targeted selective sequencing of target regions to fully and rapidly assess mutations, methylation profiles, and copy number variants in target regions using a single assay, with no additional library build process optimization, facilitating personalized panel design and analysis of samples. Please contact our staff to send your samples.