Neurotransmitter Probe Neural Stem Cell Lines

Neural stem cells (NSCs) can repair the damaged nervous system, and transplanted NSCs tend to colonize the lesion site, which holds great promise in the field of regenerative therapy of the nervous system. However, the number of NSCs is very small, and it is extremely difficult to obtain NSCs, there are ethical restrictions on obtaining NSCs from embryonic stem cell induction and differentiation. Fortunately, with the development of reprogramming technology, Alfa Cytology has achieved the direct reprogramming of adult cells into NSCs or the differentiation of induced pluripotent stem cells (iPSCs) to obtain a large number of NSCs, which has enabled the transplantation of NSCs into clinical applications.

We can provide transmonosynaptic retrograde labeling, biological labeling of neural stem cell development, and related products

Complex brain functions cannot be realized without efficient and specific information exchange and integration between neurons, and neurotransmitters, as important small biological molecules are involved in the chemical synapse-mediated information transfer process between neurons. Traditional direct measurements do not allow for the detection of transmitters at the level of individual cells and have insufficient specificity and poor temporal and spatial accuracy.

The novel probe GRAB (GPCR activation-based sensor) can accurately detect neurotransmitter dynamics in real time with high sensitivity, specificity, and resolution. We can use it to resolve complex functions of the nervous system at high temporal and spatial scales.

We form fusion proteins by inserting circularly rearranging fluorescent proteins into the region where the G protein-coupled receptor (GPCR) binds to the ligand (neurotransmitter) with the most significant conformational change. Once a specific neurotransmitter activates the GPCR to change its conformation, the conformation of the fluorescent protein linked to it also changes, and the fluorescence intensity changes, thus indicating dynamic changes in neurotransmitter concentration.

Developable neurotransmitter probe-like neural stem cells

In the nervous system, neurotransmitters and neuromodulators are mediators of cell-to-cell communication and have broad and important regulatory roles. Monitoring the concentration and dynamics of specific neurotransmitters and neuromodulators with high spatiotemporal precision can provide important information for decoding intercellular communication in neural networks in healthy and diseased states. To enhance performance, these neurotransmitter probe-like neural stem cells offered by Alfa Cytology are continuously being optimized with updated iterations. Please feel free to contact us for the latest news or related service and product inquiries.