Organoids and Organ Chip Services for Brain Tumors

Unlike traditional cells, organoids are clusters of cells with a three-dimensional spatial structure. The analysis of organoids requires both statistical analysis of a large number of cells and heterogeneous information analysis at the single-cell scale, as well as the ability to obtain information in three-dimensional space. Conventional light microscopes have a bandwidth limitation between the viewing field and imaging resolution. Therefore, the development of new 3D imaging systems for organoid analysis is an important contribution to the development of the organoid research field.

We provide organoid and organ chip and imaging services for brain tumors

Tumor organoids formed by culturing brain tumor tissues in vitro retain the biological properties of brain tumor cells in vivo to a great extent, and have the advantages of low cost and ease of operation, making up for the shortcomings of conventional brain tumor experimental models. Alfa Cytology provides organoid and organ chip services and organoid imaging services to our customers, based on our extensive experience and proven platform, aiming to closely mimic the complex structure and function of patients' brains to better show the complex pathological cellular responses and interactions.

Organoid imaging services

Drug development and cell biology research on organoid models has been at the forefront of current scientific research. We have overcome the shortcomings of conventional organoid and organ imaging to provide our clients with organoid and organ-on-a-chip imaging services for brain tumors. We have developed a 3D quantitative imaging analysis technique with the advantages of a large field of view, high resolution, real-time, and quantitative analysis, which is based on the development of a lens-free imaging device in the form of LED array scanning, and the design of 3D image reconstruction algorithms to achieve this.

The basic principle is to use lens-free imaging technology to bring the sample in close contact with a complementary metal semiconductor oxide (CMOS) chip to form a device that directly images the sample without an optical lens and to reconstruct the image by combining the principles of computational imaging.

Organoid and organ chip services

Organ chips are a more standardized system with the primary physiological function of the organ or tissue of interest and are therefore a particularly promising model for drug screening. Organ chips and organoids often use multiple cell types from their tissues. We simulate organ physiology by co-culturing cells in a supportive 3D matrix and using microfluidic channels to perfuse nutrients or compounds in the formed cellular structures and organoid technologies such as organ chips.

• We use induced pluripotent stem cell (iPSC)-derived cells or primary cells to preculture organoids.
• We transfer the cells to 24-well plates and then place them in an incubator to promote cell growth and differentiation into specific 3D tissues.
• The organoid culture process requires multiple steps and we need to perform different medium exchanges.
• We monitor and characterize the organoid to enable a complex analysis of tissue structure and differentiation.
• We perform visualization and characterization of multiple quantitative descriptors that can be used to study brain tumor phenotypes and compound effects.

Alfa Cytology can combine organoid culture and imaging services with live cell imaging, microfluidic microarrays, and bio-3D printing, which can provide new avenues for disease diagnosis, treatment option selection, and drug screening of brain tumors. Please contact our staff to learn how we can provide these services for you.