Polyclonal Antibody Development for Brain Tumors

Polyclonal antibodies are antibodies produced by the immunized host through the secretion of B cells from different sources. A group of polyclonal antibodies should recognize the same antigen and also because they are polyclonal, often recognize several different epitopes of the same antigen. There are multiple specific receptors on brain capillary endothelial cells, and by modifying the corresponding antibodies by targeting functional molecules on a nano-drug delivery system, drug transport across the blood-brain barrier can be mediated by the specific binding of ligands or antibodies to the corresponding receptors.

We offer polyclonal antibody development services for brain tumors

• Anti-CD133 polyclonal antibody preparation services

Stem cell surface proteins are commonly used to isolate and identify tumor stem cells. Therefore, the identification of specific markers on the surface of tumor stem cells is important for the diagnosis and treatment of malignant tumors, including brain tumors. CD133 can be used as a marker molecule for brain tumor stem cells, and some CD133+ tumor cells such as gliomas are closely associated with drug resistance and recurrence.

Therefore, Alfa Cytology can prepare high potency, high specificity anti-CD133 polyclonal antibodies for our customers. We use the prokaryotic expression of human CD133 extracellular region fragments as antigen immunization target animal models to determine that CD133 recombinant protein can elicit high titers of humoral immunity and to obtain anti-CD133 polyclonal antibodies.

The antibodies we provided can be used in subsequent studies to prepare anti-CD133 nanobodies, which in turn can be investigated by our customers as diagnostic reagents for the identification of brain tumor stem cells and against CD133-expressing brain tumor cells.

• CD11c polyclonal antibody preparation services

Dendritic cells are closely associated with pathogen recognition and can direct the specificity, intensity, and polarity of the immune response. CD11c is a widely used marker for dendritic cells. In diseased brains, activated microglia express CD11c.

Alfa Cytology provides our customers with polyclonal antibody products for the detection of CD11c in paraffin-embedded sections of mouse organs and PFA-fixed vibrated sections. The CD11c antibodies we offer are mouse-specific and do not cross-react with human CD11c, making them particularly suitable for differentiating murine species from human cells in humanized mouse models. And we offer CD11c antibodies that have been extensively and comprehensively tested in a large number of formalin-fixed, paraffin-embedded tissue samples.

Alfa Cytology offers polyclonal antibodies that are less expensive to prepare than monoclonal antibodies of the same species origin and have shorter preparation cycles. Because they can detect multiple epitopes of the same antigen simultaneously, they are also suitable for immunoprecipitation and enzyme-linked immunosorbent assay (ELISA). To improve the specificity of polyclonal antibodies, we generally use antigen affinity purification to obtain purified polyclonal antibodies with specificity. Please contact us to learn more about the wide range of brain tumor polyclonal antibodies we can provide for you.