Reporter Cell Line Construction Services

Reporter cell lines express detectable reporter genes, allowing our clients to monitor cellular processes, assess gene expression and evaluate the effects of therapeutic agents in real time. Alfa Cytology specializes in providing comprehensive reporter cell line construction services for brain tumor research.

Introduction to Brain Tumor Reporter Cell Line Models

Brain tumors present a significant challenge in oncology due to their complex biology and the intricacies of the central nervous system. Traditional treatment modalities often fall short, necessitating innovative research approaches to better understand tumor behavior and treatment response. Reporter cell line models serve as pivotal tools in this endeavor, enabling researchers to visualize and quantify cellular processes in real-time. These models utilize genetically modified cells that express reporter genes, which can indicate cellular events such as proliferation, apoptosis, and drug efficacy.

Fig.1 Lentiviral Transduction of Primary Human GBM Cells. (Yuzhakova D., et al., 2022)

The construction of specialized reporter cell lines tailored for brain tumor studies allows for the monitoring of tumor dynamics in response to various therapeutic agents. For instance, bioluminescent or fluorescent markers can be integrated into these cell lines, providing a non-invasive means to track tumor growth and spread in vivo. This methodology not only enhances our understanding of tumor biology but also facilitates the identification of novel therapeutic targets, ultimately improving treatment strategies.

Our Services

At Alfa Cytology, we offer comprehensive reporter cell line construction services specifically designed for brain tumor research. Our expertise encompasses the entire process, from initial design to final validation of the reporter cell lines. Alfa Cytology employs cutting-edge techniques, including CRISPR/Cas9 gene editing and plasmid-based transfection, to create custom cell lines that meet the unique needs of our clients.

• Custom Reporter Gene Selection

Options include luciferase, green fluorescent protein (GFP), and red fluorescent protein (RFP), among others. These genes can be coupled with tumor-specific promoters to enhance specificity and sensitivity, ensuring that the reporter signal accurately reflects tumor activity.

• Cell Line Development and Validation

Alfa Cytology develops stable cell lines, which include transfection, screening of positively modified cells and extensive validation to confirm reporter gene expression and function.

• Reporter Gene Functional Cell Lines

• Workflow of Our Services

Design protocols based on drug development principles

Evaluation of key signaling pathways associated with the target site

Selection of appropriate cell models based on cell characteristics

Sequence design and optimization for specific targets

• Applications
  • Studying the transcriptional activity of gene promoters. For protein blotting, protein isolation and purification, affinity chromatography, immunocytochemistry, and flow cytometry
  • Protein expression, localization, and translocation in cells. Fluorescence can be used to continuously monitor the expression levels of endogenous and recombinant proteins.
  • Target identification and evaluation of drug candidates. This can be used for brain tumor drug target identification and candidate evaluation.

Contact Us

Alfa Cytology provides services for the construction of reporter gene functional cell lines for brain tumors, which can be applied to promoter activity studies, transcription factor studies, mammalian cell two-hybrid assays, and live animal imaging experiments. Our experts will save you time and effort and ensure the smooth running of your experiments. Please feel free to contact to communicate project details.

Reference

1. Yuzhakova D., et al. (2022). Highly invasive fluorescent/bioluminescent patient-derived orthotopic model of glioblastoma in mice[J]. Frontiers in Oncology. 12, 897839.