Stable Cell Line Construction Services for Brain Tumors

Stable cell line construction is a crucial aspect of preclinical research, particularly in the study of brain tumors. These cell lines provide a reliable platform for understanding tumor biology, testing therapeutic agents, and developing novel treatment strategies. At Alfa Cytology, we specialize in creating high-quality stable cell lines tailored for brain tumor research.

Introduction to Stable Cell Line for Brain Tumor

Our Services

At Alfa Cytology, we specialize in the development of high-quality stable cell lines tailored for brain tumor research. Our comprehensive services include:

• Hybrid clonal cell lines. Gene transfection followed by direct screening with resistance drugs. The screened cells express both the resistance gene and the target gene but contain a variety of different cell clones. The location of target gene integration and expression varies between clones. It has the advantage of relatively rapid screening and low cost.
• Monoclonal cell lines. A cell line obtained from the amplification of a single cell containing a stably integrated exogenous fragment. The expression of the target gene at the site of integration is highly uniform for each cell, but some phenotypes may be lost. Monoclonal cell screens are often recommended for cell lines that require cell sublocalization experiments, are difficult to infect, and have low expression rates.

Services Flow

Some of the Stable Cell Lines that Have Been Constructed

Technical Advantages

• Excellent seed cells. Low generation and no contamination.
• Stable production process. Strict screening and target gene expression verification system.
• No loss of cell viability. Rigorous evaluation of cell proliferation viability for 5 consecutive generations after line establishment.
• Cell line quality assurance. RT-qPCR validation to ensure expression level.

Applications

• A long-term study of gene function in target cells, we can reduce the cost of frequent transfection or virus packaging by establishing stable cell lines to facilitate experimental studies.
• Some proteins have extremely long half-lives, and transient RNA can only interfere with expression and cannot remove the already expressed target protein, we can achieve better gene interference effects by building stable cell lines.
• Transient will introduce extremely high copy number expression, leading to inaccurate experimental results due to human factors, we build stable cell lines that can help to screen cells with appropriate copy numbers for experimental studies.
• Those that require an inducible expression system are mainly some lethal genes or those that require spatiotemporal expression. Those that need to use cells for animal experiments, such as nude mice tumorigenesis, etc. We can establish stable cell lines for all of them.

Alfa Cytology has rich experience in constructing and screening stable cell lines for brain tumors. We can screen high-quality stable cell lines for our customers, and you can select the appropriate cell lines from our cell bank or provide their cell lines. Please get in touch with our staff to discuss your project requirements and get more information about our brain tumor stable cell line construction and screening services.