Stem Cell Development Services for Brain Tumors

Brain tumor ranks only 10th in the incidence of adult malignant tumors, but it is one of the tumors with the highest mortality rate in human beings. Brain tumors are difficult to be completely removed by surgical treatment, and have poor sensitivity to radiotherapy, extremely high recurrence rates, and poor prognosis. In malignant brain tumors, only a very small number of tumor stem cells can proliferate indefinitely. The development of different types of brain tumor stem cells (BTSCs), the in-depth study of the biological properties of BTSCs, and the exploration of the specific role and mechanism of BTSCs in the development and recurrence of malignant brain tumors will be very important for the future search of malignant brain tumor eradication by targeting tumor stem cells.

Our BTSCs development services

BTSCs often express some surface markers of neural stem cells, such as the characteristic CD133 and Nestin proteins. Alfa Cytology offers a simple and effective method for developing BTSCs and analytical assays based on the presence of these markers. We use human brain tumor specimens, inoculated in a growth factor-free medium and cultured in a serum-free medium. The cells with the ability to form neurospheres (5%) were analyzed and identified, and the expression of CD133 and Nestin protein was obtained as positive. This method is simple and easy to use and provides a new avenue for brain tumor research.

BTSCs and their differentiated cells culture

We obtained CD133⁺ cells from tumor tissues and cultured them in serum-free DMEM/F12 medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) to form tumor spheres that grew in suspension. The tumorspheres were isolated with CD133 immunomagnetic beads to obtain CD133⁺ monocytes. And the cells were inoculated and cultured at a certain concentration. The expression rate of markers, cell proliferation cycle, and ploidy changes were also detected by flow cytometry.

We have developed different types of BTSCs such as medulloblastoma, astrocytoma, glioblastoma, and oligodendroglioma by this method. We observe the morphology, expression of differentiation-related markers, and cell proliferation kinetics during the differentiation of BTSCs in vitro, which can explain the differentiation direction of BTSCs and help you develop newer therapeutic strategies for brain tumors.

BTSCs analysis services

We can identify and analyze the biological properties of BTSCs obtained in culture. This can provide you with reliable information for further brain tumor research.

BTSCs are considered to be the seed cells of brain tumors, and only tumor stem cells can proliferate indefinitely. the presence of BTSCs is closely related to brain tumor drug resistance, therefore, Alfa Cytology provides BTSCs development methods and analysis services to support you in using BTSCs as therapeutic targets to study their resistance mechanism to radiotherapy. Please contact our staff for information on the development of the different types of BTSCs you need to accelerate your research on brain tumor growth, dissemination, recurrence, targeted therapeutic approaches, etc.