Stem Cell Development Services for Brain Tumors

Stem cell research has emerged as a cornerstone in the study of brain tumors, providing critical insights into tumor biology, progression, and therapeutic resistance. The unique properties of stem cells, including their ability to self-renew and differentiate, make them ideal models for understanding the complexities of brain tumors such as glioblastoma and medulloblastoma. Alfa Cytology specializes in stem cell development services tailored for brain tumor research.

Introduction to Brain Tumor Stem Cell Models

Brain tumors, particularly gliomas, represent some of the most challenging malignancies to treat, largely due to their complex biology and the presence of cancer stem cells (CSCs). Among these, glioma stem cells (GSCs) have garnered significant attention for their role in tumor initiation, progression, and recurrence. Understanding and modeling GSCs are crucial for developing effective therapeutic strategies.

Fig.1 Characterization of glioma stem cells. (Piper K., et al., 2021)

Stem cell models are essential for dissecting the unique characteristics of GSCs. These models allow us to study the self-renewal capabilities, differentiation potential, and tumorigenicity of GSCs in vitro and in vivo. By mimicking the tumor microenvironment, these models provide insights into how GSCs interact with surrounding cells and respond to therapies.

Applications of Brain Tumor Stem Cell Models

Our Services

Alfa Cytology offers a comprehensive suite of services focused on stem cell development for brain tumors. Our commitment to precision and innovation allows us to provide high-quality stem cell models that facilitate advanced studies in cancer biology and therapy.

• Isolation and Characterization of Tumor-Derived Stem Cells
  Using techniques such as fluorescence-activated cell sorting (FACS) and magnetic-activated cell sorting (MACS), Alfa Cytology enriches for stem cell populations. Each isolated stem cell line undergoes extensive characterization to confirm stemness markers, differentiation potential, and genetic stability, ensuring that the cells accurately represent the original tumor.
• Stem Cell Culture and Expansion
  Alfa Cytology utilizes serum-free media and specific growth factors to promote the proliferation of stem cells while maintaining their undifferentiated state.
• Functional Assays and Drug Testing
  Alfa Cytology provides a range of functional assays to investigate the biological behavior of developed stem cell lines.
  • Proliferation and Migration Assays

  • Drug Response Studies

Workflow of Our Service

Alfa Cytology obtained CD133⁺ cells from tumor tissues and cultured them in serum-free DMEM/F12 medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) to form tumor spheres that grew in suspension. The tumor spheres were isolated with CD133 immunomagnetic beads to obtain CD133⁺ monocytes. And the cells were inoculated and cultured at a certain concentration. The expression rate of markers, cell proliferation cycle, and ploidy changes were also detected by flow cytometry.

Contact Us

For researchers aiming to advance their studies on brain tumors through innovative stem cell models, Alfa Cytology is committed to providing exceptional development services. Our team of experts is ready to assist you at every step of your research journey. To learn more about our services or to discuss your specific needs, please feel free to contact to communicate project details.

Reference

1. Piper K., et al. (2021). Glioma stem cells as immunotherapeutic targets: advancements and challenges[J]. Frontiers in Oncology. 11: 615704.