Virus-induced Brain Tumor Models

Due to the discovery of a relationship between viruses and the development of brain tumors, some scholars have attempted to use viruses for brain tumor induction experiments. However, their pathogenesis has not been completely elucidated. The possible mechanism is that the virus itself has a replicative and genetic role, and upon infection of cells releases genetic material that integrates into the host cell genome and causes carcinogenesis. Some viruses carry oncogenes within their genes that encode proteins that contribute to host cell carcinogenesis.

We provide services for the building of viral-induced brain tumors model

Researchers often study brain tumors in virus-free models, but viruses may play an important role in brain tumors. Alfa Cytology can build viral-induced brain tumor models for our customers, which are well suited for studying the effects of radiation/chemotherapy in glioma. Both ribonucleic acid viruses and deoxyribonucleic acid viruses (adenoviruses) can induce brain tumors. Virus-induced brain tumors can be transmitted continuously in homozygous animals and cloned to produce biologically stable models.

The type of tumor-induced depends on the site of inoculation and the age of the animal. The convex surface of the brain or the cerebellar earthworm is susceptible to sarcoma, and the subventricular canal is susceptible to glioma. Neonatal animals are more susceptible than adult animals. From the established models of virus-induced brain tumors, it is clear that inbred animals are prone to mimic the biology of human brain tumors. We provide viral-induced brain tumors as both a model to explore the pathogenesis of brain tumors and as a source to study therapeutic models.

General services flow of virus-induced brain tumors model building

This in vitro grown cortical organoid model has a variety of neuronal cells and brain regions, and the in vivo growth and invasion of GBM is closely related to various neuronal cells. The cortical organoid model combines the advantages of three-dimensional matrix gel culture and oscillatory culture to simulate the entire process in vivo starting from embryonic stem cells, growing into the inner, middle and outer germ layers, and then differentiating into neuroepithelial tissue, neural tube structures, and cerebral cortex. The cortical organoid model can maintain the individual genotype and the expression level of functional proteins of the organ, which can be applied to human brain disease research and drug screening.

Culture of cortical organoids

• Virus preparation. The virus is cultured, propagated, released, and enriched by ultracentrifugation.
• Experimental animal preparation. Independently housed in an animal biosafety level II laboratory.
• Virus infection. The infectious dose is determined according to pre-experiments and the virus is injected after light anesthesia.
• Model analysis. The analysis indexes included symptom observation (animals were observed for 14 days after infection, and weight change, symptoms, and mortality were recorded), virus detection (major tissues and organs were isolated at a certain time after infection, RNA was extracted, and viral load in tissues was detected by real-time fluorescence quantitative PCR, and viral replication was recorded), and pathological diagnosis (major tissues and organs were isolated at a certain time after infection, and HE staining to observe pathological changes).
• Evaluation and validation of animal models.

Viruses are widely used as carcinogens because of their short latency period for tumor production and relatively high rate of brain tumor production with a wide species range. Alfa Cytology can build viral-induced brain tumor models for our customers, please feel free to contact us for your customized services.