Whole Exome Sequencing for Brain Tumors

The exome contains crucial details regarding protein synthesis, representing a direct reflection of gene functions. Whole exome sequencing (WES) employs sequence capture technology to target the exome for high-throughput gene sequencing. Alfa Cytology offers cost-effective and efficient whole exome sequencing services specially designed for brain tumors.

Whole Exome Sequencing in Brain Tumors

Whole exome sequencing (WES) stands as a remarkable breakthrough in gene sequencing, enabling the comprehensive detection of the exome across virtually all coding regions of the genome during brain tumor progression. Since the exome constitutes only a small portion of the entire genome, whole exome sequencing can obtain higher sequence depth with less raw sequence and at a reduced cost. This innovative technique widely contributes to the identification of brain tumor susceptibility genes and the catalogue of somatic mutations. Thus, whole exome sequencing represents a powerful tool for diagnosis and facilitates the discovery of novel biomarkers and potential therapeutic targets in brain tumor biology.

Fig.1 From tissue to data-steps of whole exome sequencing. (Bartha Á., & Győrffy B, 2019)

Our Services

Mutations within the exome significantly influence the onset and progression of brain tumors. Alfa Cytology offers a dependable approach to brain tumor genomic exploration by precisely identifying single nucleotide variants (SNVs) and copy number variations (CNVs) via whole exome sequencing. Our complete whole exome sequencing can efficiently pinpoint tumor susceptibility and pathogenicity genes, promoting the investigation of the underlying mechanisms of brain tumors.

Workflow of WES

Sample Types and Delivery Requirements

• Frozen fresh tissues. Fresh tissue > 5 mg, immediately frozen in liquid nitrogen for half an hour. Store at -80 °C and ship on dry ice.
• FFPE tissues. Please provide H&E slices. FFPE sections are shipped at room temperature.
• Cells. After centrifugation, collected cells > 5×10⁵ are washed twice in PBS buffer and immediately frozen in liquid nitrogen for half an hour. Store at -80 °C and ship on dry ice.
• Plasma/serum. Total volume > 2.5 mL, sodium citrate tubes or EDTA tubes are recommended. Heparin anticoagulation tubes are not recommended. Store at -80 °C and ship on dry ice.
• Blood. Total volume > 7.5 mL, sodium citrate tubes or EDTA tubes are recommended. Heparin anticoagulation tubes are not recommended. Store at -80 °C and ship on dry ice.
• gDNA. DNA dissolved in TE buffer, total volume > 300 ng, concentration 20-100 ng/μL, volume > 10 μL.

Why Choose Us?

The cost-effectiveness and efficiency of WES now stand as a distinct advantage over WGS technology. Alfa Cytology leverages WES to uncover gene-level variant insights in brain tumors such as tumor mutation load and copy number variations, aiming to facilitate the identification of predictive biomarkers and novel therapeutic targets. For further details on our whole exome sequencing services, please feel free to contact us.

Reference

1. Bartha Á., & Győrffy B. (2019). Comprehensive Outline of Whole Exome Sequencing Data Analysis Tools Available in Clinical Oncology[J]. Cancers. 11(11), 1725.