Breast Cancer single-domain antibody Development

Introduction of single-domain antibody

Nanobodies, also known as single domain antibodies or VHH molecules, are proteins found naturally in camelids that are the smallest functional fragment of a heavy chain antibody that lacks the light chain and heavy chain CH1 constant regions. single-domain antibody is one-tenth the size of traditional antibodies while maintaining strong antigen recognition capabilities. It can be used in molecular imaging, tumor diagnosis, imaging-guided surgery, cancer treatment, etc.

single-domain antibody is also widely used in the field of breast cancer (BC) research. single-domain antibody-based molecular imaging can achieve precise staging and tumor heterogeneity evaluation of TNBC. single-domain antibody directed against antigens expressed by TNBC (TNF-α, EGFR, CD3, CTLA-4, STAT3, AKT2, etc.) can be used to detect, target, or sense cancer cells.

Fig. 1. single-domain antibody production procedure. Fig. 1. single-domain antibody production procedure. (Bakherad H, et al., 2022)

Breast Cancer single-domain antibody Development Services

Breast Cancer single-domain antibodyDevelopment Services

Alfa Cytology provides single-domain antibody development services. single-domain antibody has advantages such as small size, easy production, low cost, and high stability, which make them attractive tools as cancer therapeutics or diagnostic agents.We can provide a variety of camel-derived single-domain antibody development services, including alpaca, llama, llama, llama, etc. Based on the phage display technology platform, Alfa Cytology can provide major experimental links including antigen design, alpaca immunization, library construction and screening, active function verification, etc., and provide scientists around the world with high-specificity and high-affinity single-domain antibody.

Step	Services
Alpaca immunity	A healthy adult alpaca (28-36 months) is vaccinated 5-7 times ELISA titer determination Antiserum collection
cDNA preparation (customers can provide cells)	Total RNA extraction High-fidelity RT-PCR preparation of cDNA
single-domain antibody library construction	Primer design and synthesis Using cDNA as a template, PCR amplifies the variable region of the heavy chain antibody gene Phagemid construction and transformation: enzyme digestion, ligation to phagemid vector, electroporation of TG1 host bacteria, and construction of antibody library Identification: randomly select 20-50 clones, PCR identification, sequencing, and analysis of antibody sequences
single-domain antibody library screening	Antigen extreme pressure method for 3 rounds of screening Identification: 96 clones were randomly selected and titer determined by ELISA Express 10-15 positive clones to prepare single-domain antibody supernatant

Advantages of single-domain antibody

Strong specificity, high affinity, weak immunogenicity to human body
Small molecular weight, strong tissue penetration
High solubility, strong tolerance and high stability
Simple structure, suitable for prokaryotic/eukaryotic expression systems
Modifiable

Alfa Cytology is a world-leading preclinical CRO company dedicated to assisting scientists around the world in BC research. We can provide you with BC-related single-domain antibody development services and help in the treatment and diagnosis of BC. If you have any questions about BC research, please contact us and our experts will get back to you as soon as possible.

Reference

Bakherad H, et al. Nanobodies; new molecular instruments with special specifications for targeting, diagnosis and treatment of triple-negative breast cancer. Cancer Cell Int. 2022 Aug 6;22(1):245.

All our services and products are exclusively intended for preclinical research purposes. They are not intended for diagnostic, therapeutic, or patient management applications.