Breast Cancer Organoid Modeling Services

Introduction of Breast Cancer Organoid Models

Breast cancer (BC) is the most common malignant tumor in women, and its incidence rate ranks first among female malignant tumors. Tumor organoids are the concept of combining organoids and tumors, that is, tumor cells form a microstructure similar to human tumors under appropriate culture conditions and highly restore the tissue structure and gene lineage of human tumors, which can maintain tumor heterogeneity. The construction of BC organoids is mainly composed of three elements, namely BC cells, extracellular matrix, and cytokines. BC organoids can not only promote the personalized treatment of cancer patients but also can be used for the screening of new tumor drugs and the study of the process and mechanism of BC development, etc., which has profound and revolutionary significance for the research of BC.

Fig. 1. An organoid culture protocol for ex vivo expansion of mammary stem cells isolated from mouse tissue. (Cui J, et. al., 2021)

Our Services

Alfa Cytology can select appropriate culture conditions and materials according to your needs quickly to establish BC organoid models. Our goal is to facilitate the robust and reproducible growth of organoids. From basement membrane extracts to growth factors and small molecules, we can create ideal organoid culture conditions that reduce experimental variability. The BC organoid model has the characteristics of convenient material collection, short culture cycle, and genetic stability, which can restore the tissue and molecular characteristics of BC cells in vivo to the greatest extent, and reproduce part of the mammary gland function. BC organoid models have both the advantages of genetic manipulation of tumor cell lines and the three-dimensional complex system characteristics of mouse human-derived tumor tissue xenograft models. Therefore, BC organoids have broad application prospects in the occurrence and development of BC, metastasis, and drug resistance mechanisms, related drug screening, individualized treatment, and transformation of research results.

Fig. 2. Culture protocol for breast organoids.

Advantages of BC Organoid Models

• Low cost and short cultivation period
• Genome stability was maintained after multiple passages
• Highly preserved biological characteristics and heterogeneity of orthotopic tumor tissue
• Organoids can be used to create BC organoid-derived xenografts
• Artificial control more realistic reproduction of BC tumor tissue structure

Application Fields

• Biological characteristics of BC tumor cells
• BC tumor pathogenesis and metastasis pathway
• Assess drug efficacy
• Large-scale drug screening
• Drug efficacy testing for individualized therapy in cancer patients

Alfa Cytology has many years of experience in constructing BC organoid models. Based on our advanced platform and experienced scientists, we can build customized, cost-effective, high-quality BC organoids to assist you in your BC organoid model research. If you are interested in learning more about our BC organoid services and solutions please contact us. We will give you a professional and competitively priced strategy that suits your needs as quickly as possible.