Breast Cancer RNA in Situ Hybridization

RNA in situ hybridization (ISH) is a technique used to detect and localize specific RNA molecules in breast cancer tissue samples. By visualizing RNA in its cellular context, ISH enhances the understanding of molecular mechanisms in breast cancer. Alfa Cytology offers a comprehensive suite of RNA in situ hybridization services for breast cancer research.

Introduction of RNA in Situ Hybridization

RNA in situ hybridization is a technique that uses probes such as cRNA or oligonucleotides to detect RNA expression in cells and tissues. RNA in situ hybridization detection technology is based on an innovative short fragment probe combination design and special nucleic acid protein cascade signal amplification system, taking into account specificity and sensitivity, and can detect as little as 1-10 copies of target RNA molecules on tissue sections. As long as the target RNA molecule sequence is known and longer than 100 bases, corresponding probes can be designed for detection.

Fig.1 Principles of in situ hybridization. (Veselinyová D, et al., 2021)

Our Services

Alfa Cytology can provide you with breast cancer RNA in situ hybridization service. RNA in situ hybridization can be used for qualitative, localization, and quantitative analysis in gene analysis and diagnosis, and is a very important molecular pathology technique. RNA in situ hybridization includes the basic steps of tissue cell fixation, prehybridization, hybridization, washing, and signal detection. Services we can provide include:

Services	Description	Provided by Customers	Provided by Alfa Cytology
RNA in situ hybridization	RNA in situ hybridization enables the labeled nucleic acid single strand containing a specific sequence, namely the probe, to hybridize with the complementary nucleic acid single strand in tissue cells, namely the target nucleic acid, under appropriate conditions. The labeled probe is then detected by autoradiography or immunocytochemistry to display specific RNA molecules in situ in the cell.	Sample information to be tested Provide gene name or ID, or gene sequence Sample size information and testing requirements, etc.	Stained sections Probe Sequence Electron pictures, including ordinary fluorescence microscopy or confocal laser microscopy The experimental report, including experimental instruments, reagents, methods, results, conclusions
RNA fluorescence in situ hybridization	FISH is a new in situ hybridization method formed by replacing isotope labeling with fluorescent labeling. It has the advantages of safety, rapidity, high sensitivity, long storage time of probes, and the ability to display multiple colors at the same time.

Technical Advantages

Accurate and personalized experimental plan
Comprehensive, efficient, and high-quality service experience
Low requirements on samples, experimental environment, and operation

Strong specificity and high sensitivity
Rich experimental information
Wide range of applications

Application Fields

BC drug development
BC biomarker research
BC basic science research

Alfa Cytology has many years of experience in BC histopathological analysis. Based on our advanced platform and experienced scientists, we can provide you with comprehensive, mature, reproducible, and high-quality histopathology services. If you are interested in learning about our BC histopathology analysis services, please contact us. We will give you a professional and competitively priced strategy that suits your needs as quickly as possible.

Reference

Veselinyová D, et al. Selected In Situ Hybridization Methods: Principles and Application. Molecules. 2021 Jun 24;26(13):3874.

All our services are exclusively intended for preclinical research purposes. They are not intended for diagnostic, therapeutic, or patient management applications.