Breast Cancer Scaffold Modeling Services

Introduction of Scaffold-Based Breast Cancer Model

Breast cancer (BC) is the most common cancer in women and is associated with high mortality. Compared with traditional 2D models, 3D co-culture systems represent better reproduction of the physiological conditions of in vivo interactions between tumors and stroma, leading to improved predictions. In vitro 3D models provide an efficient compromise between the biological tissue complexity that characterizes in vivo models and the experimental reproducibility found in 2D in vitro models. A scaffold-based culture system that better reflects the natural structure of cancellous bone tissue (open porosity percentage >70%) and 3D extracellular matrix than other 3D systems. Currently, in vitro scaffold-based models for BC research are still in the development stage.

Fig. 1. Schematic illustration of different types of scaffold-based 3D cell models in cancer tissue engineering. (Unnikrishnan K, et al., 2021)

Scaffold-Based Breast Cancer Modeling Services

Scaffold-based 3D cell culture methods have a long history of development. Materials used for cell culture scaffolds include basement membrane matrix, hydrogel, agarose, collagen, fibronectin, gelatin, laminin, etc. These composites mimic the natural extracellular matrix through porosity, fiber, permeability, and mechanical stability. And it can well simulate the interaction between cells and the interaction between cells and extracellular matrix in the in vivo environment, while allowing cells to aggregate, proliferate and migrate on the scaffold.

Alfa Cytology relies on its mature technology platform and professional technical team to customize the most appropriate scaffold-based breast cancer model according to the different needs of customers.

• Select and design brackets

Many factors need to be considered, including the biocompatibility, physical and chemical stability of the scaffold, etc., taking into account the complexity and practicality of the model.

• Manufacturing brackets and surface treatment

Commonly used manufacturing methods include three-dimensional printing, electrospinning, freeze-drying and solution gelling. Improve the adhesion of cells on the scaffold and enhance the bioactivity of the scaffold.

• Cell seeding

By direct contact, spray or flow culture methods.

• Cell culture and assessment

Control temperature, humidity, pH value, nutrients, etc., and regularly monitor and evaluate cell growth and differentiation.

• Model validation

Test the function and effect of the model through biological experiments, etc. Including cell survival rate, proliferation rate, differentiation rate, etc.

Alfa Cytology is a world-leading preclinical CRO company dedicated to assisting scientists around the world in BC research. We can provide you with scaffold-based BC modeling services and help in the development of therapies and diagnostics for BC. If you have any questions about BC research, please contact us and our experts will get back to you as soon as possible.