DNA Vaccine Development for Breast Cancer

Introduction of DNA Vaccine

Breast cancer (BC) is one of the major fatal cancers in women, and immunotherapy has received increasing attention as a treatment strategy for BC. DNA vaccines have emerged as an attractive approach to vaccine development against cancer and certain infectious diseases. DNA vaccines are third-generation vaccines, modern tools composed of DNA encoding proteins or specific antigens required by pathogens. The vaccine stimulates cellular and humoral responses against a variety of diseases including cancer, autoimmune diseases and infectious diseases. DNA vaccines have been shown to produce long-term cell-mediated immunity. The potency of the presence of multiple antigen genes in the same construct is one of the advantages of DNA vaccines against different types of cancer. Recombinant DNA technology plays an important role in the production of DNA vaccines. These vaccines are designed to increase DNA insertion and translation in cells, activate CD4+ T cells, and enhance cytotoxic T lymphocyte induction and antibody production. DNA vaccines are safe and have few side effects in humans and animals. Their immunogenicity can be improved by using suitable adjuvants and appropriate delivery systems.

Fig. 1. Four main antigen processing pathways may occur after DNA vaccine transfection into cells. (Pagliari S, et al., 2023)

Breast Cancer DNA Vaccine Development Services

In the development process of DNA vaccines for BC, we focus on selecting and optimizing the best antigens inserted into plasmid DNA. This is an important component and key technology in DNA vaccine development, involving antigen loss, modification and tolerance.

• DNA vaccine preparation

The preparation of DNA vaccines includes chemical synthesis of DNA encoding antigens, bacterial fermentation amplification of recombinant plasmids, plasmid isolation and purification, and finally encapsulation into nanoparticles. Our services include plasmid design and selection, development of DNA vaccine production processes, and DNA vaccine quality control.

• DNA vaccine verification

Our vaccine validation includes in vitro assays and in vivo animal testing, and provides feasibility and immunogenicity evaluation of DNA vaccines, stability analysis of DNA vaccines, and safety and effectiveness evaluation of DNA vaccines.

Advantages of DNA Vaccine

• Reduced need for live attenuated pathogens (i.e. bacteria and viruses) or recombinant proteins
• Plasmid DNA can be made in a very cost-effective way
• Plasmid DNA can be stored relatively easily

Alfa Cytology is a world-leading preclinical CRO company dedicated to assisting scientists around the world in BC research. We can provide you with BC DNA vaccine development services and help in the treatment and diagnosis of BC. If you have any questions about BC research, please contact us and our experts will get back to you as soon as possible.