Genetically Engineered Animal Models of Breast Cancer
Introduction of Genetically Engineered Animal Models (GEMMs)
GEMMs are an important tool for preclinical research on human breast cancer (BC), gene editing of animal mammary glands can model common genetic abnormalities in human breast cancer. There are generally two types of GEMMs, which are transgenic breast cancer animal models and gene knockout breast cancer animal models. Basic and translational breast cancer research relies heavily on experimental animal models. Ideally, such models for breast cancer should have a commonality with human breast cancer in terms of tumor etiology, biological behavior, pathology, and response to therapeutics, and GEEMs are often used to explore the molecular and functional characteristics of BC and the targeted therapy of BC.
Fig. 1. Schematic showing the generation of mouse tumor models for BC therapy. (Bosenberg M, et al., 2023)
Our Services
Ideal animal models play a crucial role in BC research, requiring control of specific tumor subtypes and the complex biological environment required for tumorigenesis, which is a difficult, time-consuming, and costly affair. Alfa Cytology can provide breast cancer genetic engineering animal model construction services.
- Transgenic breast cancer animal models
We targeted the expression of oncogenes in mice using mammary gland-specific promoters. Among them, MMTV-LTR is mostly used for mammary gland-specific promoters. MMTV is an important virus that causes mammary tumors in mice. Oncogenes such as Wnt-1 are highly expressed in the mammary gland, thereby inducing breast cancer.
- Gene knockout breast cancer animal models
We adopt the Cre/loxp system and use two kinds of mice, the first is mammary gland-specific Cre mice, such as WAP-Cre and MMTV-Cre mice. The second is flox mice with tumor suppressor genes, such as BRCA1 (fl/fl) mice, and E-cadherin (fl/fl) mice, which can be targeted to knock out tumor suppressor genes in mammary gland tissue to induce breast cancer by mating the two kinds of mice.
We have a systematic BC transgenic animal model platform and a professional team, which can deliver the animal model you need accurately and on time.
Application Fields
- Molecular mechanisms of BC tumorigenesis
- Pathological mechanism
- BC drug screen
Advantages of Our Services
- Scientific program design
- Strict quality control management
- Professional research team
- Rich project experience
- High-quality project services
Alfa Cytology's BC GEMMs can simulate the occurrence and development of human BC, and have very important theoretical value and clinical significance in the study of BC pathogenesis, the discovery of new drug targets, and the evaluation of preclinical drug efficacy. We can provide a variety of highly efficient, customized BC GEMMs models according to the specific needs of customers. If you are interested in our service, please contact us, our professionalism and competitive price will be the reason for you to choose us.
Reference
- Bosenberg M, et al. Mouse models for immuno-oncology. Trends Cancer. 2023 Jul;9(7):578-590.