Proteomic Analysis for Breast Cancer
Introduction of Proteomic
The proteome refers to the complete set of proteins expressed by the genome, including all proteins expressed by a cell or organism. Proteomics is a science that studies the composition and changes of proteins in cells, tissues, or organisms, taking the proteome as the research object. Essentially, it is a large-scale study of the characteristics of proteins, including protein expression levels, post-translational modifications, protein-protein interactions, etc., to gain a comprehensive understanding of disease occurrence, cell metabolism, etc. At present, proteomics is widely used in the clinical diagnosis, detection, etiological prediction, and treatment decision of breast cancer. Proteomic information can be used to classify breast cancer (BC) subtypes and stages, study BC tumorigenesis mechanisms, cancer behavior and aggressiveness, and recurrence prediction, it also plays an important role in choosing the most appropriate BC treatment.
Fig. 1. Protein structure practical workflow diagram. (Neagu AN, et al., 2022)
Breast Cancer Proteomic Analysis Services
Alfa Cytology can provide you with BC proteomic analysis services. The proteomic characterization of breast tumors is critical to understanding molecular aberrations, especially the characterization of BC-associated proteins. These proteins are considered to be a unique set of potential biomarkers associated with cancer through their loss, downregulation, overexpression, or post-translational modification, and aberrant PPIs may lead to cellular dysfunction, tumor development, and affect patient survival. To better understand the function of individual proteins and their location in complex biological systems, it is necessary to measure changes in protein abundance relative to other systems. Our services include,
| Services | Technology type | Description |
|---|---|---|
| Protein qualitative | LC-MS/MS | Through LC-MS/MS, purified samples such as IP, Co-IP, and Pull-down can be detected to identify target proteins and their interacting proteins. Protein mixtures in other types of samples can also be identified to understand protein species in a given environment. |
| Protein quantification | TMT/iTRAQ marker quantification Label-free non-standard quantity PRM target validation |
Determine the total protein content of a sample or the amount of a single protein component. |
| Protein modification | Phosphorylation modification Acetylation modification Ubiquitination modification |
Many vital life processes in organisms are regulated by spatiotemporally specific, reversible post-translational modifications. Therefore, revealing the rules of post-translational modification is an important prerequisite for analyzing the complex biological functions of proteins. |
Application Fields
- Biomarker discovery
- BC classification
- Predict efficacy
- Drug target and mechanism research
Advantages of Our Services
- High sensitivity of the experimental platform
- Complete solution
- Personalized customized analysis
- Professional team and rich experience
Alfa Cytology is a leading global CRO company. Our complete, optimized, and easy-to-operate workflows provide you with fast, accurate, and reproducible results to help you achieve your research goals. We provide personalized, innovative, complete sequencing service solutions, if you are interested in learning more about our BC multi-omic analysis services, please feel free to contact us. Our professional and patient staff will get in touch with you as soon as possible.
Reference
- Neagu AN, et al. Proteomics-Based Identification of Dysregulated Proteins in Breast Cancer. Proteomes. 2022 Oct 21;10(4):35.