Transcriptome Analysis for Breast Cancer

Introduction of Transcriptome

Transcriptomics is the study of gene expression from the RNA level. The transcriptome is the sum of all RNAs that can be transcribed in a living cell, which provides key information on gene regulation and cellular protein content. The vast majority of transcripts consist of non-coding RNAs (ncRNAs), which regulate mRNA expression and protein production. Therefore, many genes involved in breast cancer (BC) are affected by ncRNAs activity. Transcriptomic analysis has become one of the most popular platforms to identify key genes in BC pathogenesis, which may play a key role in BC diagnosis, prognosis, and treatment.

Fig. 1. Classification of ncRNAs. (Yang S, et al., 2023)

Breast Cancer Transcriptome Analysis Services

Alfa Cytology can provide you with BC transcriptome sequencing services. Transcriptome profiling of breast tumor samples has become one of the most powerful approaches in holistic oncology over the past few decades. The so-called transcriptome refers specifically to the collection of mRNA. The cDNA library obtained by reverse transcription of all mRNA in tissues or cells is sequenced by high-throughput sequencing technology, and the structure and expression level of transcripts are analyzed by counting the number of related Reads. Our services include,

• ncRNAs sequencing

ncRNAs mainly refers to IncRNA, circRNA, and smallRNA. Similar to mRNA, non-coding RNA sequencing also mainly searches for differential RNAs and enrichment pathways by designing differential comparison combinations, and combines target gene prediction to explore its regulatory mechanism.

• IncRNA sequencing

IncRNA is a type of long-chain RNA with weak coding ability and a length greater than 200nt. IncRNA sequencing is to carry out accurate IncRNA identification and target gene prediction for samples with a reference genome, and at the same time provide analysis of mRNA in the sequencing data.

• smallRNA sequencing

smallRNA is an endogenous non-coding RNA molecule usually 18-30nt in length, among which miRNA is the most widely studied, and miRNA is involved in post-transcriptional regulation of gene expression.

• circRNA sequencing

CircRNA is a special kind of non-coding RNA molecule, which forms a circular structure through covalent bonding, has conservation and tissue specificity, and plays an important role in various physiological and pathological processes.

• Whole transcriptome sequencing

Sequencing analysis of all RNA transcribed from a tissue or cell at a certain point in time or under treatment conditions. The study of the whole transcriptome includes the analysis of mRNA and ncRNAs, and can combine multiple RNA information to explore the potential regulatory network between each other.

Application Fields

• Test drug biological activity
• Discovery of new molecular targets for anticancer therapy
• Identify candidate biomarkers
• Assessing treatment resistance
• Identify BC patients who are exempt from endocrine therapy

Advantages of Our Services

• Advanced and complete sequencing platform
• Professional technical team
• Rich experience in data analysis

Alfa Cytology is a leading global CRO company. We can provide you with a personalized, innovative, and complete sequencing service solution. If you are interested in learning more about our BC multi-omic analysis services, please feel free to contact us. Our professional and patient staff will get in touch with you as soon as possible.