Cell Characterization Services
Cell characterization, which includes analysis of cell type, lineage and differentiation status, and assessment of their potency and functional properties, helps researchers understand the complex mechanisms of the tumor microenvironment and the host immune system and design precisely targeted cellular therapies. Alfa Cytology integrates a powerful combination of cutting-edge technology and expertise to help meet your diverse cell characterization needs.
Fig.1 Stem cells characterization. (Sebastião, M. J., et al., 2021)
The Role of Cell Characterization in Cell Therapy Development
During the development of cancer, cells proliferate in an uncontrolled manner and often exhibit aberrant signaling pathways. Therefore, rigorous characterization of target cancer cells and therapeutic cells is a critical process in the field of cell therapy development, which is a multidimensional assessment and comprehensive analysis of cellular properties, behaviors, and functions from the molecular, genetic, and phenotypic levels. Techniques such as flow cytometry, immunohistochemistry, genome sequencing, and functional assays have helped to elucidate the molecular characteristics, surface markers, genetic alterations, and functional capabilities of cells. In addition, emerging avenues of research, including single-cell analysis, spatial transcriptomics, and multi-omics integration, are enabling researchers to gain greater insight into cellular heterogeneity and interactions within the tumor ecosystem.
Our Services
Alfa Cytology provides cell characterization services to help researchers assess the quality, purity, identity and function of cells used in the development of cancer therapies. Our specialized services and cutting-edge technologies cover but are not limited to, the following areas, allowing for a comprehensive analysis of cells to meet your specific research needs.
Immunophenotyping
Services
Our multiparameter flow cytometry platform allows for the simultaneous detection of multiple markers and precise, comprehensive quantitative and qualitative analysis of cellular phenotypes to classify cell populations based on their specific characteristics.
Cytokine
Analysis
We use various techniques, including multiple immunoassays, flow cytometry, and protein imprinting, to measure the levels of cytokines secreted by cells to assess their immune response and function, providing valuable information to understand the potential efficacy of the cells.
Genomic and Transcriptomic
Analysis
Using technologies such as fluorescence in situ hybridization, polymerase chain reaction, and single-molecule real-time sequencing, we perform high-throughput genetic and transcriptional analyses to identify specific mutations, markers, or alterations in genes that may affect cell behavior.
Functional
Assays
Our comprehensive services include evaluating the behavior of cells in response to a variety of stimuli, such as cell proliferation, migration, invasion, and differentiation, to determine the therapeutic potential and safety of engineered or primary cellular therapies, providing reliable validation of your therapeutic candidates.
Viability and Potency
Assay
Measure cell viability and potency with assays such as MTT, ATP, or ELISA to understand the ability of therapeutic cells to survive, proliferate, and exert biological activity, which can help predict the effects of cells under various therapeutics and conditions.
Metabolic Profiling and Cellular
Imaging
Using mass spectrometry, NMR spectroscopy, and other techniques to analyze cellular energy production, substrate utilization, and overall metabolic state. Techniques such as microscopy or live cell imaging are used to observe cell morphology, behavior and interactions. This helps provide insight into cell-cell function and potential responses to therapy.
Alfa Cytology offers a comprehensive cell characterization service that allows for a thorough analysis of cell quality, purity, identity and function. With a focus on precision, accuracy, and efficiency, our services ensure the highest quality data for your cell therapy development programs. Contact us today to learn how our team of experts can support your cellular analysis needs.
Reference
- Sebastião, M. J., et al.; (2021). Stem cells characterization: OMICS reinforcing analytics. Current Opinion in Biotechnology, 71, 175-181.
! For research use only.
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