Cell Isolation Services

Cell isolation is a key technology in cancer cell therapy research, which involves the isolation, extraction, and purification of specific cell populations from complex biological samples for further analysis and manipulation. Alfa Cytology is committed to providing high-quality, meticulously isolated cells for downstream applications to facilitate the development of innovative cancer therapies.

The Role of Cell Separation in Cell Therapy Development

Cell isolation is a fundamental process in cell-based cancer immunotherapy research and involves the separation of cells of interest from a variety of complex biological samples, enabling researchers to study and manipulate them effectively. Due to the heterogeneity of T cell populations, not all T cells are suitable for cellular therapies, so in the field of cancer therapy development, the precise isolation of target cells such as tumor-infiltrating lymphocytes (TILs), mesenchymal stem cells (MSCs), and the tumor cells themselves is critical for elucidating disease mechanisms, identifying therapeutic targets, and developing innovative therapeutic strategies. Currently, the cell isolation step faces many challenges, including maintaining cell viability and function, minimizing contamination, and ensuring reproducibility.

Fig.1 Technologies for Single-Cell Isolation. (Gross, A., et al., 2018)

Our Services

Isolating and expanding cell subpopulations from major sources such as blood, biological fluids, and tissues can be a time-consuming activity, and you can outsource this project to us so that you can focus on key discoveries. Alfa Cytology has the expertise and key technologies in isolating immune cells from primary tissues, aiming to achieve the highest quality and yield of therapeutic cells.

Project Evaluation

Initial assessment of sample characteristics and study objectives.

Sample Preparation

Dissociation of tumor tissue to obtain single-cell suspensions.

Protocol Development

Development of an appropriate isolation protocol based on sample type and target cell population.

Quality Control

Verification of purity and viability of isolated cells through stringent quality control measures.

Cell Optimization

Design and execution of optimization protocols for isolated cell subpopulations.

Cell Delivery

Deliver purified cell populations ready for downstream applications such as cell culture and genetic engineering.

Our Cutting-Edge Technologies

Alfa Cytology is constantly innovating and adapting to emerging technologies to fulfill the stringent requirements for accurate cell separation. We have the following cutting-edge cell separation technologies.

• Magnetically-Activated Cell Sorting (MACS). Used for positive or negative sorting of target cell populations.
• Fluorescence-Activated Cell Sorting (FACS). For high-speed, high-precision cell sorting.
• Antibody Conjugation Technology. For developing customized reagents for specific cell surface markers.
• Advanced Microfluidics Platform. For label-free, high-throughput cell sorting.
• Automation and Robotics Integration. For streamlining workflow and ensuring reproducibility.

Our Advantages

We are committed to performing each step with the highest degree of precision, ensuring that the quality of isolated cells for downstream applications meets the following requirements.

Alfa Cytology offers a comprehensive range of cell separation services for the specific needs of cancer therapy development. Our services focus on precision and efficiency and ensure that our knowledge is constantly updated, aiming to advance the frontiers of cancer therapy research with strong expertise. To meet the diverse needs of this sector, we have developed a global network of partnerships that make these capabilities available to you wherever you are. If you are interested in our services, please do not hesitate to contact us.

For research use only.