Genetically Modified Tumor Model Development Services
Alfa Cytology is committed to providing our clients with one-stop spontaneous tumor model development services, aiming to provide reliable, reproducible and translatable models that accurately reflect human disease, accelerating the process of translating from the lab to therapy.
Introduction to Genetically Modified Tumor Model
Genetically modified tumor models are developed through genetic engineering techniques, such as CRISPR/Cas9, transgenic, and knock-in/knock-out methodologies, which allow precise modifications of oncogenes and tumor suppressor genes. This precision fosters the creation of tumor models that closely mimic the genetic and molecular landscapes of human cancers.
Fig.1 Schematic representation of DNA constructs used to generate genetically engineered mouse models (GEMMs) of human cancer. (Onaciu, A., et al., 2020)
Features of genetically modified tumor models include their ability to replicate human cancer heterogeneity, genetic instability, and microenvironment interactions. These models provide a dynamic platform for studying tumor biology, drug responses, and resistance mechanisms in a controlled environment. They are applicable across a wide range of cancer types, including but not limited to breast, lung, colorectal, and pancreatic cancers.
Our Services
Leveraging the well-established technology platform of the tumor microenvironment center, Alfa Cytology offers a wide range of genetically modified tumor models for specific types of cancers and can customize modeling solutions for specific project needs. We follow the latest research and industry standards to ensure that each model accurately reflects the biology of the respective human disease.
Types of Genetically Modified Tumor Models
We provide customers with different types of customized gene-modified tumor models, mainly including the following 4 types, to meet customers' individual needs.
Transgenic Tumor Models
These models involve inserting foreign genes into the host genome to drive oncogene expression or silence tumor suppressor genes, facilitating the study of gene-specific cancer development.
For example: HER2, Myc, RET, RAS, etc.
Knockout Tumor Models
Created by removing specific genes, these models help understand the role of tumor suppressor genes by observing the effects of their absence on tumor formation.
For example: p53, BRCA1, PTEN, APC, etc.
Knock-in Tumor Models
These involve precise gene insertion at specific loci to study the effects of specific gene mutations or oncogene overexpression in cancer development.
For example: KRAS G12D, BRAF V600E, EGFR L858R, IDH1 R132H, etc.
CRISPR/Cas9 Edited Tumor Models
Utilizing CRISPR/Cas9 technology, these models allow for precise and rapid gene editing to investigate gene functions and therapeutic targets.
For example: TP53, PD-L1, NRAS Q61K, CTNNB1 (β-catenin), etc.
Process of Genetically Modified Tumor Model Construction
Gene Selection
Identify target genes associated with cancer for modification based on current genetic databases.
Genetic Engineering
Edit the selected genes, either by knocking in or knocking out specific sequences.
Cell Line Creation
Develop stable cell lines with the desired genetic modifications for tumor induction.
Tumor Induction
Implant genetically modified cells into suitable animal hosts, typically immunocompromised mice.
Validation and Analysis
Conduct thorough validation to confirm the genetic and phenotypic accuracy of the tumor models.
With extensive experience in various cancer models and expertise in a wide range of cancer modeling, Alfa Cytology is the ideal partner to support your oncology therapeutic research. We provide customers with stock model products, model customization, and joint R&D services to provide you with the best tumor models and look forward to meeting your precise needs. Please let us know your project requirements and we will provide you with a comprehensive service from solution to report. For further inquiries and custom model development, please feel free to contact us.
Reference
- Onaciu, A., et al.; (2020). Spontaneous and induced animal models for cancer research. Diagnostics, 10(9), 660.
For research use only.
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