Humanized Mouse Model Development Services
Focusing on the development of humanized mouse models, Alfa Cytology is committed to precise model customization and stringent quality control, aiming to more accurately replicate human tumor biology, thereby improving predictive power in preclinical studies.
Introduction to Humanized Mouse Model
Humanized mouse models are genetically engineered mice that carry functional human genes, cells, tissues, or organs. By incorporating human immune system components, humanized mouse models can more accurately replicate the human immune response to cancer therapies, offering a more relevant and predictive platform for evaluating new treatments.
Fig.1 Humanized immune system mouse models. (Allen, T. M., et al., 2019)
Applications of Humanized Mouse Model
Humanized mouse models can be engineered to express specific human genes or harbor human immune cells, allowing them widely used in preclinical cancer research.
- Used to identify potential biomarkers for treatment response and resistance.
- Used to evaluate the pharmacodynamics and pharmacokinetics of new drugs, understand tumor biology and the tumor microenvironment, and investigate the mechanisms of immune evasion by cancer cells.
Our Services
With a well-established technology platform of the tumor microenvironment center, Alfa Cytology provides our clients with one-stop solutions for pretreated PDX model development. Our team of expert scientists can tailor the perfect preclinical efficacy testing strategy for your specific research needs.
Humanized Mouse Models Available
Alfa Cytology provides a diverse range of humanized mouse models tailored to different cancer types, including but not limited to:
| Types of Cancer |
Humanized Mouse Models Available |
| Lung cancer |
Non-small cell lung cancer (NSCLC) humanized mouse models; Small cell lung cancer (SCLC) humanized mouse model. |
| Breast cancer |
Estrogen receptor-positive (ER+) breast cancer humanized mouse model; Triple-negative breast cancer humanized mouse model. |
| Colorectal cancer |
Colorectal (CRC) humanized mouse model; Models of microsatellite stable (MSS) CRC models. |
| Melanoma |
Cutaneous melanoma humanized mouse model; Uveal melanoma humanized mouse model. |
Analysis Services
Alfa Cytology also provides analytical services, including preclinical pharmacology studies and efficacy evaluation of new therapies using humanized mouse models.
In Vivo Studies
To understand the absorption, distribution, metabolism, and excretion (ADME) of investigational drugs, as well as their biochemical and physiological effects.
Biomarker Discovery and Validation
Utilizing advanced molecular and genomic techniques, to identify and validate the biomarkers predictive of drug response and disease progression.
Genomic and Transcriptomic Analysis
Advanced genomic technologies are utilized to analyze genetic and transcriptomic changes induced by drug treatments, to understand the mechanisms of action, identifying resistance pathways.
Molecular Imaging and Functional Assays
Functional assays, including flow cytometry, ELISA, and Western blotting, complement imaging studies by providing detailed molecular and cellular insights.
Service Process of Humanized Mouse Model Development
Initial Consultation and Planning
Detailed planning to determine the appropriate humanized mouse model and required genetic modifications.
Validation and Characterization
Extensive characterization through phenotypic and genotypic analyses to ensure accuracy and reproducibility.
Full-Scale Study Implementation
Providing ongoing support and data analysis to ensure successful study outcomes.
Model Development and Genetic Engineering
Incorporating human genes, cells, or tissues as specified in the research plan.
Pilot Studies and Optimization
Making necessary adjustments to enhance model performance and relevance.
Alfa Cytology provides comprehensive solutions to address key challenges encountered in the field of cancer therapy development. With extensive experience in a wide range of cancer models and expertise in tumors and microenvironments, we are committed to providing our clients with the most accurate in vivo cancer models to meet their precise needs. For inquiries or to learn more about how we can support your cancer research program, please contact us.
Reference
- Allen, T. M., et al.; (2019). Humanized immune system mouse models: progress, challenges and opportunities. Nature immunology, 20(7), 770-774.
! For research use only.
Related Services
