Chromosomal Translocations Detection Development Services

Chromosomal translocations are established genetic drivers in leukemia pathogenesis. Comprehensive gene structure analysis enhances understanding of leukemia's biological mechanisms and accelerates diagnostic development. Alfa Cytology delivers specialized chromosomal translocation analysis for leukemia research. Leveraging advanced technologies including fluorescence in situ hybridization (FISH) and Next-Generation Sequencing (NGS), we provide high-precision analytical services to advance your leukemia pathogenesis studies.

Overview of Chromosomal Translocations in Leukemia

Chromosomal translocations are fundamental molecular events in leukemogenesis, serving as both disease mechanisms and diagnostic biomarkers for hematologic malignancies. These genetic rearrangements involve non-homologous chromosome exchange, generating fusion genes that produce novel oncogenic proteins. Such fusion proteins dysregulate critical cellular processes—including proliferation, differentiation, and apoptosis—driving leukemic transformation. For instance, the t(8;21)(q22;q22) translocation is a well-established leukemia driver. Clinically, translocation detection enables precise diagnosis, targeted therapy development, prognosis assessment, and disease monitoring in leukemia.

Fig. 1. Acute promyelocytic leukemia is associated with the chromosomal translocation t(15;17)(q22;q21). (de Almeida, T.D.; et al., 2023)

Alfa Cytology provides comprehensive chromosomal translocations detection development services for leukemia, including but not limited to the following.

Chromosome Karyotype Analysis

To provide our services, we utilize the Giemsa dye for the visualization of chromosomal translocations in the standard karyotype analysis of leukemia. By carefully observing chromosome behavior during cell division, we can identify phenomena such as chromosome breakage, rearrangement, and translocation.

Detection of Chromosome Translocation in Leukemia by PCR

As part of our comprehensive services, we offer RT-PCR analysis to detect fusion genes associated with leukemia. Through our advanced genetic testing techniques, we have the capability to accurately detect and identify specific gene rearrangement events resulting from chromosomal translocations.

FISH Analysis for Leukemia Chromosomal Translocations

We can offer you the FISH analysis technique, which uses specific fluorescent probes to observe and locate chromosomal translocations. These probes can bind to specific sequences to provide visual information on chromosomal translocations. We also provide spectral karyotype analysis technology to further deepen the study of chromosome translocations in leukemia.

Chromosome Banding Analysis for Leukemia

We utilize Giemsa staining for G-banding technology. This technique enhances the visualization and detailed analysis of chromosomes, allowing us to provide relevant and valuable information regarding chromosomal translocations in leukemia.

Whole Genome Sequencing for Leukemia

Leveraging next-generation sequencing technology, our whole genome sequencing service offers a more comprehensive understanding of chromosomal translocations. By sequencing the entire genome, we can detect and identify global changes associated with chromosomal translocations, enabling us to determine the specific locations and impacts of these events.

Nanopore Sequencing for Leukemia

We offer nanopore sequencing as part of our services. Nanopore sequencing enables us to successfully identify the exact location of chromosomal breakpoints, yielding efficient, accurate, and comprehensive experimental results. This cutting-edge technology empowers us to provide clients with precise insights into the structural alterations caused by chromosomal translocations in leukemia.

Alfa Cytology is committed to the field of hematologic oncology, especially leukemia research. The chromosomal translocation detection technology we provide can be applied to the study of blood tumors to analyze the loci information in the genetic variation, which can bring efficient, fast, and accessible solutions to customers. If you would like to know more about our services, please contact us.