Feline Leukemia Virus Detection Development Services
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Feline Leukemia Virus Detection Development Services

While the removal of infected animals and the development of effective vaccines have helped mitigate feline leukemia virus (FeLV) to a certain extent, it remains a significant pathogen in domestic cats. Testing experimental animals for feline leukemia virus can provide valuable insights for decision-making in affected individuals. Alfa Cytology is dedicated to the research of leukemia viruses and serves as a provider of high-quality products and services. We prioritize customer needs and offer tailored solutions to address the challenges posed by FeLV.

Introduction to Feline Leukemia Virus

The most effective method for preventing feline leukemia is to screen blood samples for the virus and isolate infected cats. Over the years, detection of FeLV infection has become more advanced and now includes diagnostic assays that assess various viral and immunologic parameters. The most commonly used methods involve detecting free FeLV p27 antigen, viral RNA, and proviral DNA. Proper detection of FeLV is crucial for accurately interpreting test results and determining the FeLV status of the cat.

Molecular detection of vector-borne pathogens in cats tested for FIV and FeLV.Fig. 1. Molecular detection of vector-borne pathogens in cats tested for FIV and FeLV. (de Melo, T.B. et al., 2023)

Our Services

To test samples for the presence of infection and to determine the stage of FeLV infection, Alfa Cytology develops assays that quantify FeLV-specific nucleic acid and protein levels. We offer a diverse range of biological techniques and methods to enhance the accuracy and reliability of test results.

Development of Immunochromatography for FeLV Detection

The p27 antibody was employed to identify the presence of the p27 antigen in blood and other secretions, enabling the detection of FeLV infection in the sample. We offer standard immune methods for FeLV detection and can also customize the testing approach based on the provided sample. We offer several types of immunochromatography assays to detect FeLV antigens and antibodies in samples.

  • ELISA
  • Immunofluorescence
  • Virus Isolation Techniques

Development of Molecular Methods for FeLV Detection

By utilizing a PCR-based method, we can precisely detect the presence of proviral DNA. Our PCR assays have been designed to target the U3 region of the 5' long terminal repeat (LTR), allowing detection of the infection even in the absence of viral replication or viremia. We have developed multiple molecular methods to accurately detect FeLV DNA provirus or RNA, ensuring the provision of precise experimental results.

  • Conventional PCR
  • Real-time quantitative PCR
  • Nested PCR
  • Recombinase polymerase amplification

Advantages and Disadvantages of Two Technologies

Technologies Advantages Disadvantages
Immunochromatography Rapid, easy, convenient False negative, specific stages of the disease
Molecular Methods Accurate, sensitive Time-consuming, required instrument

Advantages of Our Services

  • We have a team of experienced researchers and scientists specializing in the field of feline leukemia.
  • We take a comprehensive approach to address the challenges of feline leukemia.
  • We offer customization options to tailor our services according to the specific needs of our clients.
  • We utilize state-of-the-art technology and advanced laboratory techniques in our research and development processes.

Alfa Cytology provides a one-stop service for feline leukemia. By choosing our Development of Detection for Feline Leukemia Virus services, you can benefit from accurate and timely diagnosis, allowing for effective management and control of FeLV. Collaborate with us to discover innovative solutions for addressing the challenges posed by the feline leukemia virus. Feel free to contact us and discuss your specific research requirements.

Reference

  1. de Melo, T.B.; et al. Molecular detection of vector-borne pathogens in cats tested for FIV and FeLV. Veterinary Parasitology: Regional Studies and Reports. 2023, 40: 100857.
For research use only. Not intended for any clinical use.