Mechanism of Action Studies Services for Leukemia
The investigation of the mechanism of action (MoA) poses a significant challenge during the development of therapeutic small molecule compounds. Alfa Cytology specializes in offering mechanism of action research services to numerous prominent biotechnology and pharmaceutical companies, as well as research institutions. Our services aim to identify the specific biochemical interactions through which candidate drugs exert their pharmacological effects.
Introduction to Mechanism of Action
The mechanism of action for specific molecules typically involves interactions with biological macromolecules such as enzymes and receptors. In leukemia, Etoposide is an integral component of combination chemotherapy. The therapy-associated leukemia caused by it is characterized by chromosomal rearrangements involving the MLL gene located on chromosome 11q23. Additionally, it quinone inhibits CREBBP or TCPTP.
Fig. 1. The underlying mechanism of etoposide induced therapy-related leukemia. (Zhang, W. et al., 2021)
Understanding the mechanism of action for drug compounds is essential for successful drug development and prevention of late-stage failures during the approval process. The Mechanism of Action Studies Services we provide encompass various aspects, assisting you in addressing challenges encountered in leukemia drug development projects.
Compound Binding
Demonstrating target engagement is a crucial step in determining the mechanism of action for a compound. We offer services to measure the binding of compounds to specific protein targets, validating whether the drug reaches the target organ or tissue and whether it interacts with the intended target.
Functional Analysis
We offer top-quality services to identify drugs that can effectively and selectively modulate target protein functions. Through our services, we can accelerate your research on target identification and mechanism of action in drug discovery. Our services facilitate your understanding of the mechanism of action of drugs.
Metabolome Analysis
Metabolomics, as a systematic approach, can play a role in the study of drug mechanisms of action. Investigating the related metabolites before and after drug treatment helps reveal changes in endogenous metabolites induced by the drug and explore the drug's role in such regulation.
Pathway Analysis
We possess a toolkit for pathway analysis, which relies on knowledge of biological molecular functions, statistical tests, and other algorithms. Using statistical methods with various models, we aid our clients in identifying the most relevant pathways in a given experiment.
Methods of Determining the Mechanism of Action
We employ diverse complementary approaches to identify complex mechanisms of action. Utilizing state-of-the-art tools and highly integrated methods, we investigate small molecule drugs with unknown cellular targets. These methods encompass a range of techniques, including biophysical assays, biochemical analysis, computational analysis, multiomics analysis, RNAi screening, and gene expression profiling.
- Biophysical Assays
- Computational Analysis
- RNAi Screening
- Biochemical Analysis
- Multiomics Analysis
- Gene Expression Profiling
Alfa Cytology provides professional mechanism of action studies services, dedicated to helping clients comprehensively understand the mechanisms of drugs, compounds, or biomolecules. If you are interested in our Mechanism of Action Studies services or have any inquiries, please feel free to contact our team. We look forward to collaborating with you and providing the best mechanistic analysis services for your research.
Reference
- Zhang, W.; et al. Etoposide, an anticancer drug involved in therapy-related secondary leukemia: Enzymes at play. Translational Oncology. 2021, 14(10): 101169.