Cell models play an important role in the pancreatic cancer therapy study. Co-cultured cell models can more closely reproduce the tumor microenvironment to simulate the in vivo environment and obtain more realistic data. Alfa Cytology uses extensive expertise and cutting-edge technology to develop highly repeatable and reliable coculture cell models that can be used to gain valuable insights into tumor occurrence, progression, and therapy response.
Overview of Coculture Multiple Cells
Co-culture multiple cell system offers new ways to study cancer, developmental biology, and tissue engineering. The co-culture of endothelial cells and smooth muscle cells enables the vascular structure to be imitated. The co-culture of cancer cells and intratumor stromal cells plays an important role in understanding pancreatic cancer genesis, development, and progression. However, a significant challenge with co-culture methods is the inability to simulate ECM accurately. This shows the need for technologies that support building complex ECM-like 3D scaffolds.
Fig. 1 Different cell co-culture strategies. (Vis MAM., et al., 2020)
There is debate about the advantages and challenges of cell culture, especially traditional monoculture and co-culture systems. Coculture consists of two different cell types, rather than a single cell type, which can freely interact in the culture. Both approaches have their areas of application in pancreatic cancer therapy development. However, given that natural tissues have a complex and dynamic composition and multicellular interactions, understanding how various cell types communicate with each other is valuable for developing effective therapies, as co-culture systems can provide the best tissue-specific microenvironment for in vitro studies.
Our Services
Co-culture is a method of growing two or more different types of cells in the same cell culture container. This practice allows for direct communication and interaction between groups of cells to study various biological phenomena. Alfa Cytology offers a variety of co-culture programs to choose from.
By Method
- Using chemical agents, such as mitomycin, to prevent mitosis and co-culture adherent cells after mitosis, or seed them in pre-set proportions
- Coculture different types of cells in the same cell culture container
- Utilizing three-dimensional co-cultured spheres, such as multicellular tumor spheres (MCTS) models
In addition, choosing the right medium is critical, as it can significantly affect cell-cell interactions in a co-culture system. It is essential to adjust the composition of the medium to best reflect the physiological environment to be studied.
By Cell Type
A key feature of pancreatic cancer is a dense pro-fibroblastic response, which plays an important role in metastasis and therapy resistance. Therefore, studies of tumor-matrix interactions in vitro co-culture models usually focus on cancer cells and fibroblasts. At the same time, we also offer other customized co-culture models for various project research purposes, such as immune activation.
- Human pancreatic cancer cell and fibroblast
- Macrophages and patient-derived cancer cells
- Pancreatic tumor cell and stellate cell
- Pancreatic cancer cell and T cell
By Application
- Study of tumor growth, tissue regeneration, and adaptive immune system
- Study of intercellular crosstalk
- To simulate an environment in which multiple cell types can communicate
- Understand the entire tissue and potential drug interactions
Why Choose Us?
Scientific Experience
Professional team of scientists and more than ten years of experience in pancreatic cancer
Customized Service
Tailored services dedicated to ensuring customer satisfaction
Data Security
Strictly keep confidential the client's project information and experimental data
Quick Reply
Our customer service representatives are available 24 hours a day from Monday to Sunday
Each type of cell model has its advantages and limitations, and using a combination of several models can more comprehensively study drug resistance in pancreatic cancer and develop new treatments. If you are interested in our services, please contact us for more details. You can contact our staff directly and receive professional, reliable, and fast feedback.
Reference
- Vis MAM, et al. Impact of Culture Medium on Cellular Interactions in in vitro Co-culture Systems. Front Bioeng Biotechnol. 2020;8:911. Published 2020 Aug 4. doi:10.3389/fbioe.2020.00911
