In Vitro PK Services

Pharmacokinetic (PK) testing is critical to understanding metabolism and potential drug interactions in the early pancreatic cancer therapy development stages. As a leading specialist in non-clinical PK and toxicology services, Alfa Cytology is committed to providing our clients with cost-effective, repeatable, high-quality data.

Introduction to ADME and PK

Understanding the absorption, distribution, metabolism, excretion (ADME), and PK properties is critical for selecting candidates with desirable properties in the later stages of therapy development. The rate and extent to which the active drug enters the systemic circulation, i.e. the bioavailability, is influenced by many factors, including solubility, permeability, protein binding, and metabolism.

Our Services

Alfa Cytology has an in vitro experimental system service platform to evaluate drug absorption, distribution, metabolism, and excretion. By focusing on ADME and PK data of pancreatic cancer therapy, our integrated services can improve the properties of lead compounds and predict and select highly exploitable therapeutic candidates with good bioavailability and safety. We perform automatic effectiveness screening in the lead optimization process and provide services in the following areas.

Physiochemical Properties

Understanding the physicochemical properties of therapeutic drugs is critical for formulation development and in vivo absorption and distribution studies.

• Water solubility

• Lipophilicity

• Stability

Absorption and Permeability

Absorption refers to the process of pancreatic cancer therapeutic drugs entering the body to play their role, which is affected by many factors such as dosage form, physical and chemical properties, and administration route. The permeability of drugs on the biofilm is a key factor affecting absorption and distribution. Drugs can cross cell membranes by passive diffusion, assisting passive diffusion, active transport, and pinocytosis.

• Caco-2 permeability
• Dermal absorption
• Transporter substrate identification

• MDCK permeability
• Parallel artificial membrane permeability assay (pampa)

Distribution

Drug distribution refers to the reversible movement of drugs from one location to another in the body. In theory, different body parts could receive different doses and drugs stay for different durations. The distribution of drugs in the body depends on many things. For example, the physicochemical properties, permeability, tissue, and plasma protein binding ability.

• Brain junction
• Plasma protein binding

• Microsomal binding
• Plasma separation

In Vitro Metabolism

After the pancreatic cancer therapeutic drug enters the body for some time, it is eliminated by excretion or metabolism into one or more metabolites. Metabolism usually occurs in the liver, and in vitro drug metabolism is measured by the activity of enzymes contained in the cytochrome CYP450 superfamily.

• Metabolite analysis and identification

• Metabolic stability determination

• Drug interaction (DDI) detection

ADME Screening

It is important to evaluate drug metabolism and optimize drug ADME properties in the early stages of drug development. To avoid unnecessary losses, Alfa Cytology has established reliable in vitro ADME high-throughput screening models to provide screening and analysis services for conventional compound screening.

Plate management of test compounds Optimization of the MS/MS method In vitro ADME experiments and sample clean up Collection and reduction of the raw LC-MS/MS data Archival of the processed ADME data

Why Choose Us?

Customized solution Highly-experienced expert team Variety of preclinical models Quick turnaround time Worldwide service

With experts experienced in pancreatic cancer therapy development, Alfa Cytology is committed to providing customers with the most reliable, feasible, and effective results at a competitive price. We can meet our customer's needs and achieve research objectives. Please contact us for more information about our services.