O6-Benzylguanine

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Catalog No.: PC0178

Size: 5 mg, 10 mg, 25 mg,50 mg, 100 mg, 200 mg, 500 mg

Description

O6-Benzylguanine is a guanine analogue and inhibitor of the DNA repair enzyme O6-alkylguanine-DNA alkyltransferase (MGMT/AGT). O6-benzylguanine acts as an AGT substrate and transfers its benzyl group to the AGT cysteine residue, thereby irreversibly inactivating AGT and preventing DNA repair. O6-benzylguanine induces tumor cell apoptosis.

Molecular Weight	241.25
Formula	C12H11N5O
CAS No.	19916-73-5
Appearance	Solid
Color	White to off-white
SMILES	NC(N=C1OCC2=CC=CC=C2)=NC3=C1NC=N3
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
*In Vitro*	The L3.6pl cells are relatively sensitive to O6-Benzylguanine (24-72 hours) in a dose- and time-dependent manner. The IC₅₀ is 50 μg (at 48 hours). O6-Benzylguanine (50 μg; 48 hours) modulates p53 downstream target protein expression, induces apoptosis, and decreases cell proliferation. O6-Benzylguanine (50 μg; 48 hours) significantly decreases the MGMT transcriptional activity in L3.6pl.
*In Vivo*	O6-Benzylguanine (100 μg; i.p.; daily for 35 days) inhibits pancreatic cancer cell growth and increases pancreatic cell sensitivity to Gemcitabine (100 mg/kg). O6-Benzylguanine inhibits pancreatic cancer cell proliferation and induces tumor cell apoptosis in vivo.

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