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Pancreatic CSC Isolation and Culture
In the pancreatic cancer (PC) tumor microenvironment, cancer stem cells (CSCs) are similar to normal stem cells, with self-regeneration, heterologous replication, and anti-apoptotic functions, which may be related to high aggressiveness. Alfa Cytology offers CSC isolation and culture services to obtain purified and stable CSCs. Our services provide an experimental basis for our global customers in developing therapeutic options for pancreatic CSC.
Introduction to Pancreatic Cancer Stem Cell
The mortality rate of pancreatic cancer is rising rapidly. It is predicted that by 2030, pancreatic cancer will be the second leading cause of cancer-related death in the United States. Current evidence suggests that in highly malignant tumors, CSCs are not only responsible for the generation of a complex, differentiated tumor cell lineage, but also have the potential to contribute to the ineffectiveness of radiotherapy. Pancreatic CSCs (or stem-like cells) have been identified through various cellular and animal models as potential cancer-inducing cells capable of initiating new tumor tissue.
Fig. 1 Pancreatic CSCs features. (Valle, S.; et al., 2018)
As a new and potential research hotspot in cancer research, many scientists need to find and identify tumor cell populations with stem cell-like properties as a possible source of metastasis and treatment resistance. However, the challenges lie in identifying pancreatic CSCs and understanding their specific mechanisms.
Our Services
As one of the deadliest cancers, pancreatic cancer is characterized by rapid invasion and metastasis, but there is currently no effective therapy. Inhibition of pancreatic CSCs and their downstream effects may provide a novel and effective strategy for pancreatic cancer therapy. Alfa Cytology has an in-depth understanding of primary pancreatic cancer cell and CSC assays. We offer pancreatic CSC isolation and culture services to help our global clients answer important preclinical questions about drug candidate compounds and other therapies. We can help researchers and professionals achieve many goals, including but not limited to the following.
- High yield, high purity isolation of pancreatic CSCs (or stem-like cells)
- Identification of CSCs from human pancreatic cancer cell lines with flow cytometry (FCM)
- Assessment of the invasive and metastatic potential of pancreatic CSCs
- Confirming the stem-like characteristics of pancreatic CSC
- CSC marker identification and validation
- Genomic analysis of pancreatic CSCs with highly sensitive sequencing and qPCR technologies
Our Technology Platforms
Our pancreatic CSC isolation and induction services are supported by state-of-the-art infrastructure and market-leading technology.
- Confocal microscopy
- Scratch assay
- Flow-sorting methodology
- Clonogenic and sphere-forming assay
Why Choose Us?
Scientific Experience
Professional team of scientists and more than ten years of experience in pancreatic cancer
Customized Service
Tailored services dedicated to ensuring customer satisfaction
Data Security
Strictly keep confidential the client's project information and experimental data
Quick Reply
Our customer service representatives are available 24 hours a day from Monday to Sunday
Alfa Cytologyprovides optimized services for pancreatic CSC research, ranging from identification and culturing to downstream applications, such as genomic and functional analysis. We use the most trusted and widely referenced cell culture products and technologies to meet our customers' CSC needs. If you are interested in our services, please contact us. We are looking forward to working on your next projects!
Reference
- Valle, S.; et al. The Ever-Evolving Concept of the Cancer Stem Cell in Pancreatic Cancer. Cancers 2018, 10, 33. https://doi.org/10.3390/cancers10020033
