RIN-5F

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Catalog No.: PC0018

Description

RIN-5F is a secondary clone of the rat islet tumor cell line RIN-m (Sigma catalog number 95071701). Cloning was performed by limiting dilution in RPMI 1640, 10% FBS, and 20% conditioned medium. Conditioned medium contains growth hormone and prolactin produced by rat pituitary tumor cells GH3. RIN-5F cells secrete insulin, produce high levels of the key APUD enzyme, and contain membrane-bound secretory granules.

Origin	Rat islet cell tumour
Application	Study of pancreatic islet cell biology eg. synthesis, storage and secretion of insulin
Biological Source	Rat pancreas
Growth Mode	Adherent
Karyotype	Not specified
Morphology	Not specified
Products	Insulin
Receptors	Not specified
Technique	Cell culture, suitable for mammals.
Relevant Disease	Cancer
Shipped In	Dry ice
Culture Medium	RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

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