Scaffold-Based 3D Tumor Model Development Services
Scaffold-based 3D tumor models offer a physiologically relevant approach to studying cancer by providing structural support and mimicking the extracellular matrix (ECM) environment of tumors. At Alfa Cytology, we leverage biomaterials and scaffold fabrication techniques to create highly representative 3D tumor models that closely mimic the architecture, cellular composition, and mechanical properties of tumors in vitro.
Introduction into Scaffold-based 3D Tumor Modeling
The tumor microenvironment plays a critical role in cancer progression, metastasis, and therapeutic resistance. Traditional two-dimensional (2D) cell culture systems fail to replicate the complex interactions between tumor cells, stromal components, and the ECM, leading to limitations in translational relevance and therapeutic efficacy. Scaffold-based 3D tumor models address these limitations by providing a more physiologically relevant platform for cancer research.
Fig.1 Scaffold-based 3D cell culture models. (Abuwatfa, W. H., et al., 2024)
Scaffolds are an important component in 3D cell culture systems as they provide a 3D environment for cells to grow and interact with each other and their surroundings. Biomaterials used in such models can be categorized into the following main groups: polymer scaffolds, hydrogels, decellularized tissue scaffolds, and hybrid scaffolds.
Common Scaffold-based 3D Cell Culture Matrices
| Scaffold Type |
Description |
| Natural polymers |
They are derived from natural sources such as collagen, fibrin, or alginate. These scaffolds mimic the native ECM and provide a favorable microenvironment for cell growth. |
| Synthetic degradable polymers |
They are fabricated using synthetic biodegradable materials such as polycaprolactone (PCL) or poly (lactic-co-glycolic acid) (PLGA). These scaffolds offer precise control over their properties and can be tailored to specific requirements. |
| Hydrogels |
Composed of a 3D network of hydrophilic polymer chains capable of retaining large amounts of water. They provide a hydrated and soft environment for cell growth. |
| Decellularized tissues |
It involves the removal of cellular components from native tissues, leaving behind the ECM structure and bioactive molecules. These scaffolds provide an environment that closely resembles the native tissue microenvironment. |
| Scaffold-based microfluidics |
Cells are cultured within microscale channels that provide a controlled microenvironment for cell growth in a cell-laden scaffold. |
Our Services
Our Scaffold-Based 3D Tumor Model Development Services encompass a range of techniques and approaches, including the use of natural or synthetic scaffolds, 3D printing technologies, and tissue engineering principles. By carefully selecting biomaterials and optimizing scaffold fabrication methods, we create 3D tumor models with enhanced structural integrity, cell-cell interactions, and ECM remodeling capabilities.
Workflow of Our Service
Initial discussions with clients to understand research objectives and model requirements.
Sourcing of relevant cell populations followed by optimized culture conditions to maintain viability and functionality
Cultivation of assembled models under controlled conditions to promote maturation and tissue-like behavior.
Interpretation of experimental data and summarization of findings for further refinement and dissemination.
Careful selection and preparation of biomaterials tailored to the specific needs of the 3D models.
Seeding of cells onto prepared biomaterials and assembly into 3D structures to mimic tissue architecture.
Comprehensive analysis to evaluate structural, functional, and phenotypic properties of the development of 3D models.
Alfa Cytology is a leader in scaffold-based 3D tumor model development, dedicated to advancing cancer research and therapeutic development through innovative and cutting-edge technologies. With a multidisciplinary team of experts, state-of-the-art facilities, and a commitment to excellence, we empower researchers and pharmaceutical companies to accelerate the pace of discovery and translation in the fight against cancer. Contact us today to learn more about our Scaffold-Based 3D Tumor Model Development Services and how we can support your research endeavors.
Reference
- Abuwatfa, W. H., et al.; (2024). Scaffold-based 3D cell culture models in cancer research. Journal of Biomedical Science, 31(1), 7.
! For research use only.
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