Scaffold-Free 3D Tumor Model Development Services
Scaffold-based 3D tumor models offer a physiologically relevant approach to studying cancer by providing structural support and mimicking the extracellular matrix (ECM) environment of tumors. At Alfa Cytology, we leverage biomaterials and scaffold fabrication techniques to create highly representative 3D tumor models that closely mimic the architecture, cellular composition, and mechanical properties of tumors in vivo.
Introduction into Scaffold-free 3D Tumor Modeling
The tumor microenvironment plays a critical role in tumor initiation, progression, and response to therapy. Scaffold-free 3D tumor models aim to recapitulate the complex interactions between tumor cells, stromal components, and the extracellular matrix (ECM) without the use of exogenous scaffolds. By allowing cells to self-assemble and organize into three-dimensional structures, these models provide a more accurate representation of the in vivo tumor microenvironment, including cell-cell interactions, nutrient gradients, and ECM remodeling.
Common Scaffold-free 3D Cell Culture Models
| Non-scaffold-based Technique |
Description |
| Anchored spheroids / hanging-drop spheroids |
They are also known as hanging-drop spheroids. Cells are suspended in a droplet of media that is hung from a surface. The droplet provides a 3D environment for cells to grow and form spheroids. Anchored spheroids do not require a scaffold or matrix to support the cells. Typically, they can be maintained for 10 to 14 days. |
| Non-adherent / ultra-low attachment spheroids |
It is also known as a multicellular tumor sphere formation assay. Cells form tumor spheres through anchorage-independent growth mechanisms. Typically, these spheres can be maintained for up to 7 days. |
| Scaffold-free bioprinting |
Involves the direct printing of cell aggregates or individual cells without using an external scaffold. In this method, the cells are printed in a way that allows them to self-assemble and interact with neighboring cells, forming 3D tissue-like structures without the need for a supporting scaffold. Typically, they can be maintained for 2 to 4 days. |
| Magnetic levitation |
It is a relatively new scaffold-free method for 3D cell culture. In this method, cells are mixed with magnetic nanoparticles and levitated using a magnetic field. As the cells levitate, they aggregate and form 3D structures. Magnetic levitation allows for the creation of complex 3D structures. Typically. The cell culture can be maintained for more than 7 days. |
| Scaffold-free microfluidics |
Fosters 3D cell culture environments without supporting matrices, such as hydrogels or scaffolds. These devices create controlled microenvironments within microfluidic channels to facilitate the aggregation and self-organization of cancer cells, promoting the formation of 3D structures, including spheroids or organoids. |
Scaffold-free methods have become particularly useful in high-throughput screening due to their small size and easily modified platform. It has been found that patient-specific tumor spheroids retain their genome over time, enabling high-throughput screening and thus providing relevant data for drug screening in a patient-specific manner.
Fig.1 Methods of scarffold-free 3D tumor. (Anthon, S. G., & Valente, K. P., 2022)
Our Services
Our Scaffold-Free 3D Tumor Model Development Services offer researchers a versatile and customizable platform for studying various aspects of tumor biology and evaluating therapeutic interventions. Whether it's spheroids, organoids, or multicellular aggregates, we provide tailored solutions to meet the specific needs and research objectives of our clients.
Workflow of Our Service
Consultation and Project Design
Cell Sourcing and Culture
Culture and Maturation
Data Analysis and Reporting
Biomaterial Selection & Preparation
Cell Seeding and Model Assembly
Characterization and Validation
Alfa Cytology is a leader in scaffold-based 3D tumor model development, dedicated to advancing cancer research and therapeutic development through innovative and cutting-edge technologies. With a multidisciplinary team of experts, state-of-the-art facilities, and a commitment to excellence, we empower researchers and pharmaceutical companies to accelerate the pace of discovery and translation in the fight against cancer. Contact us today to learn more about our Scaffold-Based 3D Tumor Model Development Services and how we can support your research endeavors.
Reference
- Anthon, S. G., & Valente, K. P. (2022). Vascularization strategies in 3D cell culture models: from scaffold-free models to 3D bioprinting. International Journal of Molecular Sciences, 23(23), 14582.
! For research use only.
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