T cell-based assays offer a comprehensive understanding of T cell biology, immune responses, and their implications in diseases such as cancer. At Alfa Cytology, we offer innovative T cell-based assays, contributing to scientific advancements in the field.
Introduction to T Cell Based Assays
T cells are pivotal players in the immune response against cancer, but their phenotype and functional profile can be severely compromised in the tumor microenvironment due to various mechanisms of immune evasion employed by the tumor. An in-depth investigation into the intricacies of T cell dynamics is critical to uncovering the complex nature of cancer immunopathogenesis. By exploring these distinct T cell populations and their unique characteristics, we can gain a deeper understanding of their impact on tumor immunity and develop more precise therapeutic interventions.
Fig.1 Peripheral T cell fates after antigenic activation. (Waldman, A. D., Fritz, J. M., & Lenardo, M. J., et al., 2020)
Our Services
At Alfa Cytology, we are excited to offer our expertise in T cell-based assays, providing biology experts with valuable insights into the complex world of T cell biology and immune responses. Our services include, but are not limited to:
Cytokine Response Assays
Our cytokine response assays allow for the quantification of cytokine production by T cells upon stimulation. By measuring the levels of key cytokines such as interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), and interleukins (IL-2, IL-4, IL-6), we can provide insights into the immune response and the potential efficacy of immunotherapeutic interventions.
T Cell Proliferation Assay
Our T cell proliferation assay enables the assessment of T cell division and expansion in response to specific antigens or stimuli. By measuring cell proliferation markers such as carboxyfluorescein succinimidyl ester (CFSE) dilution or incorporation of thymidine analogs, we provide quantitative data on T cell activation and clonal expansion.
T Cell-Mediated Cytotoxicity Assay
By co-culturing T cells with labeled target cells and measuring the release of cytotoxic molecules or the induction of apoptosis, we provide quantitative data on T cell-mediated killing. We aid our clients in understanding the potency of T cell responses, identifying immune evasion mechanisms employed by cancer cells, and evaluating the efficacy of immunotherapies targeting T cell function.
Immune Checkpoint Assays
Dysregulation of immune checkpoints can lead to immune evasion by cancer cells. By assessing markers such as programmed cell death protein 1 (PD-1), cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), and lymphocyte-activation gene 3 (LAG-3), we can provide insights into the immunosuppressive mechanisms employed by tumors and aid in the development of novel checkpoint inhibitor therapies.
Alfa Cytology recognizes the significance of T cell-based assays in advancing immuno-oncology research. With a commitment to providing customized services, Alfa Cytology empowers clients to tailor their experiments, leading to more precise and meaningful scientific discoveries. If you are interested in our service, please contact us for more details.
Reference
- Waldman, A. D., Fritz, J. M., & Lenardo, M. J. (2020). A guide to cancer immunotherapy: from T cell basic science to clinical practice. Nature Reviews Immunology, 20(11), 651-668.
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