In Vitro Modeling Services for Bladder Cancer
In vitro modeling involves the cultivation and manipulation of bladder cancer cells or tissues in a controlled laboratory environment outside of living organisms. In vitro models serve as valuable tools for hypothesis testing, drug screening, and mechanistic investigations. At Alfa Cytology, we provide our clients with comprehensive in vitro modeling services for bladder cancer.
Introduction of In vitro Models for Bladder Cancer
In vitro modeling offers several advantages in bladder cancer research. Firstly, it provides a controlled and reproducible experimental setting, allowing researchers to precisely manipulate and monitor specific variables. In vitro models also offer a cost-effective alternative to animal models, reducing the reliance on animal testing and ethical concerns associated with such studies.
Advantage and deficiency of in vitro models are shown below:
|
Source |
Advantage |
Deficiency |
| In vitro 2D models |
Cell lines from human, mouse, rat and dog. |
- Easy to culture;
- Economic;
- Readily available;
- Easy to manipulate;
- Mouse and rat cell lines can be implanted into immunocompetent host to study immunotherapy;
- The most widely used model in BC research.
|
- Different genetic and epigenetic compositions from those of human cancers because of long term in vitro culture;
- Relatively pure cancer cell population
- Lack of supporting cells and TME
- Human cancer lines can only be implanted into immunocompromised mice and are not suitable to study immunotherapy
|
| In vitro 3D models |
Organoids |
- 3D structure with supporting cells and TME;
- Similar genetic alterations as the parental cancer cells;
- Relatively high concordance of drug sensitivity with parental cancers;
- Cheaper and easier than in vivo models.
|
- Lack of in vivo factors, such as blood circulation
- Lack of dynamic immune system
- Special medium with growth factor support
|
| 3D printing |
- 3D structure with supporting cells and TME;
- Precise control of cancer cells, stromal cells and stroma;
- Rapid production of large numbers of tumor 3D printings.
|
- Need for special 3D printer
- Lack of in vivo factors
- Difference of 3D structure in 3D printing compared to native cancers
- Lack of dynamic immune system as in vivo
|
| Bladder Cancer-On-A-Chip |
- In vivo relevant physiological microenvironment
- Vasculature-like perfusion
- Precise control of chemical gradient flows
- Mechanical forces
- Fully personalized system
|
- High expertise barrier
- High time-and resource-consuming
- Short-term culture
- Limitations in high-throughput analyses
- Patient availability
|
Our Services
At Alfa Cytology, we are at the forefront of providing comprehensive preclinical contract research organization (CRO) services in the field of bladder cancer, with a specific focus on in vitro model development. Some of the key services we offer include:
Contact Us
In vitro modeling services for bladder cancer provided by Alfa Cytology offer a comprehensive and cutting-edge approach to advance research and development in this field. With our expertise, state-of-the-art technologies, and commitment to scientific excellence, we aim to support researchers and pharmaceutical companies in their quest for improved diagnostics, novel therapeutics, and personalized treatment strategies for bladder cancer.
If you are interested in our in vitro modeling services, please contact us today.
Reference
- Zhu S., Zhu Z., and et al. Preclinical Models for Bladder Cancer Research. Hematol Oncol Clin North Am. 2021, 35(3): 613-632.
For research use only. Not intended for any clinical use.
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