Scaffold-Based Bladder Cancer Modeling Service
Three-dimensional (3D) cell scaffolds are designed to mimic the natural extracellular matrix (ECM) in bladder cancer tissues and organs, supporting cell growth and organization in a three-dimensional environment. Alfa Cytology offers bladder cancer modeling services based on various material scaffolds.
3D Cellular Scaffold Models for Bladder Cancer
3D cell culture systems are becoming increasingly popular in bladder cancer research, tissue engineering, drug discovery, and drug resistance studies. In comparison to conventional two-dimensional (2D) cell culture models, the 3D cellular scaffold model presents several notable benefits. It creates a microenvironment that more accurately reflects physiological conditions by enabling cells to grow in 3D, departing from the limitations of flat surfaces. Consequently, this closer approximation to the native tissue architecture allows for enhanced cell-cell and cell-matrix interactions, closely resembling those observed in vivo.
Fig.1 Major differences between two-dimensional (2D) and three-dimensional (3D) culture systems of cancer cells. (Farouk S. M., et al. 2023)
The 3D cellular scaffold model also facilitates the study of complex cellular behaviors and processes, including cell migration, proliferation, differentiation, and response to stimuli or drugs. It allows researchers to investigate tissue development, disease progression, and therapeutic interventions in a more accurate and representative manner.
Our Services
Alfa Cytology offers bladder cancer modeling services based on various material scaffolds. These scaffolds are designed to create a three-dimensional (3D) environment that mimics the tumor microenvironment and facilitates the study of bladder cancer biology, drug responses, and therapeutic interventions. Here are some of the material scaffolds utilized by Alfa Cytology in their bladder cancer modeling services:
| Type of Scaffolds |
Advantages |
Disadvantages |
 |
Hydrogels |
- Tissue-like responsiveness.
- Water-soluble factors are easily supplied to cells.
- Generally biocompatible.
- Low immunogenicity.
|
- Mechanical resistance is minimal.
- Physically cross-linked gels are weak.
|
 |
Decellularized scaffolds |
- Provides ECM environment.
- High bioactivity.
- Low immunogenicity.
- Promotes cell-material interactions.
|
- Decellularization of thick tissues can be difficult.
- The number of cell adhesion sites is limited.
|
 |
Fibrous scaffolds |
- Characterized by high surface-area-to-volume favoring cell proliferation, migration, adhesion and differentiation of cells
|
- Low structural stability
- Limited by cell seeding
- Scaffold morphology is difficult to regulate.
- Limited in thickness and small pore size.
|
 |
Microsphere scaffolds |
- Cumulative release of encapsulated bioactive substances
- Long-time maintenance of cancer cells in culture
- Excellent mechanical properties
|
- May results in loss of bioactivity of encapsulated factors.
- Residual solvent toxicity.
- Expensive.
|
 |
Nanoparticle incorporated scaffolds |
- Tunable surface properties
- High penetration ability
|
|
Contact Us
At Alfa Cytology, we are dedicated to providing comprehensive preclinical CRO services in the field of bladder cancer. To learn more about our scaffold-based bladder cancer modeling services or to discuss your specific research requirements, please contact us.
Reference
- Farouk S. M., Khafaga A. F., and Abdellatif A. M. Bladder cancer: therapeutic challenges and role of 3D cell culture systems in the screening of novel cancer therapeutics. Cancer Cell Int. 2023, 23, 251.
For research use only. Not intended for any clinical use.
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