Cell Line Development Services for Astrocytoma

Astrocytes provide structural and metabolic support to the brain and neurons, modulate ion concentrations in the extracellular gaps, regulate synaptic transmission, and uptake and release neurotransmitters. They are an integral part of the blood-brain barrier. Astrocytomas are primary brain tumors that originate from astrocytes. Although a small number of low-grade astrocytomas are benign, the majority of these tumors are malignant and often lead to a poor prognosis.

We offer services for astrocytoma cell line development

Cell lines are often used as astroglioma models because they are easier, faster to perform, and less costly. Alfa Cytology' astrocytoma cell line development service can help you find valuable biomarker molecules for brain astrocytoma and, hopefully, identify candidate targets for gene therapy of malignant brain astrocytoma, providing a new strategy for gene therapy of brain malignant astrocytoma.

Development flow

Our cell lines were developed through the steps of sampling, constructing (primary and passaged culture by tissue block culture method and single cell culture method), and cell characteristics observation.

• Cell morphology. Light microscopy morphology and transmission electron microscopy are present.
• Cell growth characteristics. Cell growth curve, cell division index, plate colony effect.
• Chromosome analysis. After hypotonicity, fixation, filming and drying, staining, and light microscopic examination, it was observed that the chromosome morphology was significantly abnormal and had the characteristics of malignant tumor cells.
• Immunocytochemical staining for astrocyte marker protein (GFAP). It was positive, proving that it was indeed a malignant tumor of astrocytic origin.

Successfully developed astrocytoma cell lines

The astrocytoma cell line we provide is a mature adnexal fibroblast cell line obtained from human brain astrocytoma tissue after shearing, digestion, culturing, passaging, and identification. It can be used for in vitro studies of astrocytoma cells.

Advantages

• Can be used for subcutaneous tumor formation in mice.
• Passes bacterial, fungal, mycoplasma, and endotoxin assays.
• Pass cell recovery viability assay, recovery survival rate >90%.
• Pass STR assay.

Isolation and identification of human astrocytoma stem cells

We additionally provide human astrocytoma stem cell isolation and identification services to form a complete cell line development system. We use the direct culture method to isolate and culture astrocytoma stem cells. We observe their proliferation and differentiation and perform immunocytochemical identification of nestin and CD133, and immunocytochemical identification of neuron-specific enolase, glial fibrillary acidic protein, and O4 after induction of differentiation.

• Isolation and culture of tumor stem cells. Observation of tumor stem cell growth in primary and passaged cultures.
• Clone formation assay. Single-cell inoculation for 5-7 d to form clone spheres.
• Identification of tumor stem cells. Immunocytochemical staining showed cells were immunopositive for nestin, CD133.
• Induction of differentiation in vitro. Adjacent cell protrusions were interconnected to form a network between cell balls, and differentiated cells had multiple morphology and obvious heterogeneity.

Alfa Cytology offers astrocytoma cell line development to provide a better cellular model for the investigation of the mechanism of carcinogenesis, observation of tumor biology, drug screening, monoclonal antibodies against astrocytoma-associated antigens, and preparation of lead drugs. Please feel free to contact our staff for details so that we can customize an astrocytoma cell line to meet your research needs as soon as possible.