CHAT Polyclonal Antibody

CAT#: NEM-K001

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Overview Specification

Target	CHAT
Cell Type	Motor neurons
Host	Rabbit
Isotype	IgG
Applications	WB, IP, IHC, IF, FC, ELISA
Reactivity	Human, mouse, rat
Conjugate	Unconjugated
Keywords	Motor neurons; Rabbit; IgG; WB, IP, IHC, IF, FC, ELISA

Product Information	It targets CHAT in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Background Information	CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons. It has been observed that CHAT gene generates multiple mRNA splice variants, including peripheral type CHAT (pCHAT) and common type CHAT (cCHAT), encoding 40-55 kDa and 66-70 kDa forms of proteins, respectively. This antibody can recognize both of pCHAT and cCHAT.
Synonyms	CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O acetyltransferase, CMS1A, CMS1A2
Full Name	Choline acetyltransferase
Tested Reactivity	Human, mouse, rat
Type	Antibody
Class	Polyclonal
Immunogen	Peptide
Calculated Molecular Weight	83 kDa
Observed Molecular Weight	47-50 kDa, 66-70 kDa
GenBank Accession Number	NM_020549
Gene ID	1103
Gene Symbol	CHAT
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage.
Positive WB Detected	Mouse brain tissue, mouse placenta tissue, human placenta tissue, Jurkat cells, rat brain tissue
Positive IP Detected	Rat brain tissue
Positive IHC Detected	Mouse cerebellum tissue, mouse brain tissue, rat brain tissue, human lung cancer tissue
Positive IF Detected	Mouse brain tissue

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.

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