Epigenomics Analysis for Brain Tumors
Epigenetic modifications refer to reversible and heritable alterations in gene expression that occur without changes to the underlying DNA sequence. While genotype remains the same, alterations in phenotype could lead to abnormal gene expression or silencing of tumor suppressor genes which then contribute to the onset of brain tumors. At Alfa Cytology, we offer specialized and professional epigenomics analysis services tailored to brain tumors.
Overview of Epigenomics
Epigenomics encompasses the thorough investigation of epigenetic changes throughout the entire genome, including DNA methylation, histone modification, nucleosome remodeling and the shaping of three-dimensional chromatin structures. Through enhancing or reducing the transcription of nearby or distal genes, epigenetics plays a pivotal role in regulating antitumor immune responses. Given their potential in the early phases of carcinogenesis before genetic mutations occur, epigenetic pathways are viewed as a promising approach for early interventions against brain tumors.
Fig.1 Therapeutic strategies to modulate the epigenome. (Hogg SJ., et al., 2020)
Targeting Epigenetics for Brain Tumor Therapy Development
Dysregulation of epigenetic features is common in brain tumor progression. Recent studies have demonstrated that epigenetic regulation is crucial for anticancer immune response and a variety of epigenome targeted drugs have been developed for brain tumors, focusing on DNA methylation, histone post-translational modifications, chromatin structure modulation and noncoding RNA regulation.
| Targets |
Drug Types |
| DNA Methylation |
|
| Histone Modifications |
- HDAC inhibitors
- BET inhibitors
- EZH2 inhibitors
- LSD1 inhibitors
|
Our Services
Alfa Cytology employs cutting-edge technologies to uncover the underlying molecular mechanisms of brain tumors and are committed to advancing novel therapeutics. Our professional epigenomics analysis services include but are not limited to the following choices.
DNA Methylation Analysis
Comprehensive DNA methylation analysis tailored to your requirements is provided.
- Whole genome bisulfite sequencing (WGBS)
- Reduced representation bisulfite sequencing (RRBS)
- Bisulfite amplicon sequencing (BSAS)
- Targeted bisulfite sequencing
- Methylation-specific PCR (MSP)
Histone Modification Analysis
To explore covalent modifications of histone tails, a comprehensive suite of histone modification analysis services is offered.
- Chromatin immunoprecipitation sequencing (ChIP-Seq)
- Histone modifications assays (acetylation, methylation, phosphorylation, ubiquitination and SUMOylation)
Chromatin Accessibility Analysis
Multiple analyses of chromatin accessibility are offered to provide insights into epigenetic landscapes.
- Assay for transposase-accessible chromatin using sequencing (ATAC-Seq)
- DNase I footprinting assay
Chromatin Conformation Analysis
Proficient analysis of three-dimensional chromatin interactions is conducted to understand chromatin architecture and its influence on gene expression.
- Chromosome conformation capture sequencing (3C-Seq)
Furthermore, Alfa Cytology also offers an extensive range of RNA epigenomics sequencing services to investigate non-coding RNA (ncRNA)-associated regulatory mechanisms throughout tumor progression. Our suite of assays includes RNA immunoprecipitation sequencing (RIP-seq), methylated RNA immunoprecipitation (MeRIP) and RNA bisulfite sequencing.
Workflow of Epigenomics Analysis for Brain Tumors
Sample Preparation
Epigenomic Sequencing
Library Construction
Data Analysis
Advantages of Our Epigenomics Analysis
- Accurate epigenomics determination. We possess a track record of reliable library quality supported by extensive experience in sample processing and library construction built upon years of accumulated expertise.
- High resolution analysis. We offer high-resolution and high-fidelity chromatin conformation services to support your exploration of brain tumor biomarkers and therapeutic targets.
- Comprehensive service package. Our comprehensive quality control epigenomics services encompass a full suite of analyses to meet your various requirements.
Contact Us
Equipped with cutting-edge technologies, specialized expertise and in-depth data analysis, we empower researchers to delve into DNA methylation, histone modification, nucleosome remodeling and the shaping of three-dimensional chromatin structures. Alfa Cytology offers a gateway to investigating the impact of epigenetic modifications and identifying prospective therapeutic targets tailored to brain tumor research. For further details on our services, please feel free to contact us.
Reference
- Hogg SJ.; et al. (2020). Targeting the epigenetic regulation of antitumour immunity[J]. Nature reviews. Drug discovery. 19(11), 776–800.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
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